Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), DNA Microscopy (DNA Mic)
Specificité
This antibody recognises KIR2D members of the killer cell immunoglobulin (Ig)-like receptor (KIR) family, CD158a, CD158b and P50.3. Clone NKVFS1 specifically recognises the long and short forms CD158a and CD158b (KIR2DL, KIR2DS1 and KIR2DS2 respectively) and also p50.3 (KIR2DS4). The Clone is reported to have Functional Activity, activating NK cell cytotoxicity via KIR2DS and inhibiting via KIR2DL forms. We recommend the use of Azide Free antibody Cat.-No SM2036A for this purpose.
Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. ELISA. Immunoprecipitation. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4, 0.09 % Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
KIR2D family members are cell surface glycoproteins with two Ig domains, which are expressed on natural killer cells and some T cells.Synonyms: CD158a, CD158b, CD158i, KI2DL1, KILLER CELL Immunoglobin-Like Receptors, KIR2DL3, KIR2DS4