CD8 anticorps
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- Antigène Voir toutes CD8 Anticorps
- CD8
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Reactivité
- Rat
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD8 est non-conjugé
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- This antibody recognises the CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells. Clone MRC OX-8 is suitable for use in in vitro blocking studies. We recommend the use of SM259A for this purpose. This clone has been described reacting with paraffin-embedded material following PLP Fixation (periodatelysine paraformaldehyde)
- Réactivité croisée (Details)
- Species reactivity (tested):Rat.
- Purification
- Affinity chromatography on Protein G
- Immunogène
- Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
- Clone
- OX-8
- Isotype
- IgG1
- Top Product
- Discover our top product CD8 Anticorps primaire
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- Indications d'application
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Flow Cytometry: 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen and paraffin sections: This product does not require protein digestion pre-treatment of paraffin embedded sections, This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.2, 0.09 % Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- CD8
- Autre désignation
- CD8 (CD8 Produits)
- Synonymes
- anticorps CD8, anticorps Leu2, anticorps MAL, anticorps p32, anticorps CD8a molecule, anticorps CD8A
- Sujet
- The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
- ID gène
- 24930
- NCBI Accession
- NP_113726
- UniProt
- P07725
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