MHC Class I RT1.Aa alpha-Chain (RT1.AA) anticorps

N° du produit ABIN120722

Détail du produit

Antigène: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Purification: Protein G affinity chromatography

Immunogène: Rat spleen glycoproteins

Clone: OX-18

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: use 10 μL of 1/50 - 1/100 diluted antibody to label 10^6 cells in 100 μL. (This product is routinely tested in flow cytometry on rat splenocytes). Immunohistochemistry on frozen sections: Acetone fixation is recommended - the antigen is sensitive to fixation with paraformaldehyde. Not suitable for immunohistochemistry on paraffin sections. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: MRC OX-18 has been used in immunoaffinity purification of rat MHC class I molecules.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Référence

Sakurada, Osawa, Araki: "[Aortic root replacement and pulmonary vein isolation, report of a case]" dans: Kyobu geka. The Japanese journal of thoracic surgery, Vol. 61, Issue 2, pp. 153-6, (2008) (PubMed).

Détail du antigène

Antigène: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Autre désignation: MHC Class I (RT1Aa) (RT1.AA Produits)

Synonymes: anticorps RTA, anticorps Rt1.aa, anticorps RT1 class I, locus A, anticorps RT1-A

Sujet: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: RT1 class I histocompatibility antigen AA alpha chain