Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func)
Specificité
This antibody recognizes Interferon beta (chIFN-beta). This antibody does not cross react with IFN gamma. This antiserum neutralizes chIFN-beta and can be used to discriminate IFN alpha and IFN beta bioactivities.
Immunoprecipitation. Western Blot: This antibody detects a band of approximately 20 kDa. Funktional Assays. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
PBS
Agent conservateur
Azide free
Stock
-20 °C
Stockage commentaire
Store the antibody at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Reports suggest that chIFN exhibits properties similar to mammalian IFN type I (IFNI). Functional studies revealed the presence of two distantly related IFNI genes, designated as IFN alpha and IFN beta, found on chicken chromosome Z. The serological distinction between these two IFNs has been verified using neutralisation assays, and there is evidence that the antiviral activity of chIFN-beta is approximately tenfold less compared to chIFN-alpha. This would suggest that chIFNbeta might have a different physiological role, though chIFN-beta is expressed strongly in virus-infected cells.Synonyms: Fibroblast interferon, IFB, IFN-beta, IFNB1