ELISA, Western Blotting (WB), Functional Studies (Func)
Specificité
The antibody 1696 recognizes free N-terminus of mature HIV protease (HIV-1 and HIV-2), an enzyme that hydrolyzes polyproteins of HIV viruses into functional proteins. The antibody 1696 does not react with the precursor.
Functional application: The antibody 1696 strongly inhibits the enzyme activity of HIV-1 and HIV-2 proteases. Western blotting: Recommended dilution: 0.5 μg/mL.
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.4
Agent conservateur
Azide free
Conseil sur la manipulation
Do not freeze.
Stock
4 °C
Stockage commentaire
Store at 2-8°C. Do not freeze.
Dunn, Boyer, McWilliams, Smith, Hughes: "Mutations in human immunodeficiency virus type 1 reverse transcriptase that make it sensitive to degradation by the viral protease in virions are selected against in patients." dans: Virology, Vol. 484, pp. 127-35, (2015) (PubMed).
Dunn, Boyer, Clark, Hughes: "Mutations in HIV-1 reverse transcriptase cause misfolding and miscleavage by the viral protease." dans: Virology, (2013) (PubMed).
Bartonová, Král, Sieglová, Brynda, Fábry, Horejsí, Kozísek, Sasková, Konvalinka, Sedlácek, Rezácová: "Potent inhibition of drug-resistant HIV protease variants by monoclonal antibodies." dans: Antiviral research, Vol. 78, Issue 3, pp. 275-7, (2008) (PubMed).
OLoughlin, Greene, Matsumura: "Diversification and specialization of HIV protease function during in vitro evolution." dans: Molecular biology and evolution, Vol. 23, Issue 4, pp. 764-72, (2006) (PubMed).
Lescar, Brynda, Rezacova, Stouracova, Riottot, Chitarra, Fabry, Horejsi, Sedlacek, Bentley: "Inhibition of the HIV-1 and HIV-2 proteases by a monoclonal antibody." dans: Protein science : a publication of the Protein Society, Vol. 8, Issue 12, pp. 2686-96, (2000) (PubMed).
Antigène
HIV Protease
Classe de substances
Viral Protein
Sujet
The HIV protease (PR) hydrolyzes polyproteins of HIV virus into functional protein products that are essential for its assembly and subsequent activity. This maturation process occurs as the virion buds from the host cell. HIV protease inhibitors are used in the treatment of patients with AIDS and were considered the first breakthrough in over a decade of AIDS research. HIV protease inhibitors can lower the viral load carried by AIDS patents.,HIV-1 PR, HIV-2 PR