ZAP70 anticorps (C-Term)

N° du produit ABIN125756

Détail du produit anti-ZAP70 anticorps

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Épitope: C-Term

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ZAP70 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Specificité: The antibody ZAP-03 reacts with ZAP70, a 70 kDa protein tyrosine kinase expressed in T and NK cells (intracellular antigen). ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP70 with histidine tag

Clone: ZAP-03

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: Intracellular staining, recommended dilution: 2-5 μg/mL, positive control: HPB-ALL human peripheral blood T cell leukemia cell line.
Western blotting: Recommended dilution: 0.5-2 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-ZAP70 (ABIN125756)

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." dans: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).

Détails sur ZAP70

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Autre désignation: ZAP-70 (ZAP70 Produits)

Synonymes: anticorps SRK, anticorps STCD, anticorps STD, anticorps TZK, anticorps ZAP-70, anticorps 70kDa, anticorps AI327364, anticorps Srk, anticorps mrtle, anticorps mur, anticorps zap70, anticorps MGC80670, anticorps MGC88962, anticorps ZAP70, anticorps zgc:110383, anticorps zeta chain of T-cell receptor associated protein kinase 70, anticorps putative tyrosine-protein kinase zap-70, anticorps zeta-chain (TCR) associated protein kinase, anticorps zeta chain of T cell receptor associated protein kinase 70, anticorps zeta-chain (TCR) associated protein kinase 70kDa, anticorps zeta chain of T cell receptor associated protein kinase 70kDa L homeolog, anticorps zeta chain of T cell receptor associated protein kinase 70kDa, anticorps tyrosine-protein kinase ZAP-70, anticorps ZAP70, anticorps Smp_149470, anticorps Zap70, anticorps zap70.L, anticorps zap70, anticorps LOC707555, anticorps LOC100342021, anticorps LOC100390922, anticorps LOC100542877

Sujet: Zeta chain of T cell receptor associated protein k,The ZAP70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP70 gene mutations resulting in lower ZAP70 protein expression levels or expression of catalytically inactive ZAP70 proteins, have been identified. ZAP70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency.In patients with chronic lymphocytic leukemia (B-CLL), ZAP70 expression on B cell was shown to be correlated with disease progression and survival. ZAP70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP70 on Tyr493 in the activation loop. ZAP70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.,ZAP-70, ADMIO2

ID gène: 7535

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway