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PRDX6-PS2 anticorps (C-Term)

PRDX6-PS2 Reactivité: Arabidopsis thaliana ELISA, WB Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN125822
  • Antigène
    PRDX6-PS2 (Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))
    Épitope
    C-Term
    Reactivité
    Arabidopsis thaliana
    Hôte
    Lapin
    Clonalité
    Polyclonal
    Application
    ELISA, Western Blotting (WB)
    Réactivité croisée (Details)
    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
    Attributs du produit
    Expected / apparent Molecular Weight of the Antigene: 25 / 20 kDa
    Purification
    serum
    Immunogène
    thioredoxin fusion of 58 amino acid region between Ser at position 175 and the C-terminal Ala of AtGPX7 accession At4g31870 has been used for antibody production
  • Indications d'application
    1: 5000 with alkaline phosphatase system (AP) (WB), 1:1, 1:2, 1:4, 1:8, 1: 16 (ELISA)
    Commentaires

    This antibody is recognizing Arabidopsis thaliana GPX (two chloroplastic isoforms). Antibody has also a weak cross-reactivity to cytosolic forms, therefore work on isolated chloroplasts is recommended.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 100 μL of sterile water.
    Conseil sur la manipulation
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Stock
    -20 °C
    Stockage commentaire
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Lepistö, Pakula, Toivola, Krieger-Liszkay, Vignols, Rintamäki: "Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods." dans: Journal of experimental botany, Vol. 64, Issue 12, pp. 3843-54, (2013) (PubMed).

    Kim, Jin, Yoon: "Decarbonylated cyclophilin A Cpr1 protein protects Saccharomyces cerevisiae KNU5377Y when exposed to stress induced by menadione." dans: Cell stress & chaperones, (2010) (PubMed).

  • Antigène
    PRDX6-PS2 (Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))
    Autre désignation
    GPX
    Sujet
    AGI Code: At4g31870
    GPX belongs to the group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). Glutathione peroxidase catalyzes the reduction of H2O2, organic hydroperoxides, and lipid peroxides in the presence of glutathione, the hydrogen donor. Activity of the enzyme can be increased under environmental stress. Immunogen: Peptide target chosen from C-terminal of two chloroplastic isoforms of Arabidopsis thaliana GPX.
    Poids moléculaire
    expected: 25 kDa, apparent: 20 kDa
    UniProt
    Q9SZ54
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