EGFR anticorps (pTyr869)
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- Antigène Voir toutes EGFR Anticorps
- EGFR (Epidermal Growth Factor Receptor (EGFR))
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Épitope
- pTyr869
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Reactivité
- Humain, Souris
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp EGFR est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificité
- Clone 12A3 specifically recognizes EGFR phosphorylated at Tyrosine 869 and detects EGFR activation after interaction with src kinases. Antibody AM00031PU-N does not crossreact with the highly homologues pTyr 877 of activated erbB2.
- Réactivité croisée (Details)
- Species reactivity (tested):Human, Mouse.
- Attributs du produit
- Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1
- Purification
- Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
- Ingrédients
- incl. positive Control
- Immunogène
- Phosphopeptide conjugated to KLHEpitope: Phosphotyrosine 869AA Sequence: E K E pY H A E
- Clone
- 12A3
- Isotype
- IgG1
- Top Product
- Discover our top product EGFR Anticorps primaire
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- Indications d'application
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ELISA: Use at 0.1 μg/mL (direct ELISA). Immunocytochemistry: Use at 1-10 μg/mL. Immunohistochemistry on Frozen and Paraffin Embedded Material. Immunoprecipitation: Use at 1-10 μg per 10^6 vanadate-treated A431 cells. Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer (cat#AS00002BU-N). Included Positive Control: Cell lysate delivered with this product (See Protocol below! ).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocole
- Positive control cell lysate provided: HepG2 EGF Treated - recommended for immunoblottingFormulation: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min. withEGF. Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGEsample buffer, mix and incubate at 90°C for 5 min. Store in aliquots at -20°C. Avoidrepeated freezing and thawing. Applications: 20 µl of positive cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (mini gel) forHRPO/ECL detection of the target proteins.
- Restrictions
- For Research Use only
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- Reconstitution
- Restore in 1 mL distilled water for 15 min at RT.
- Buffer
- PBS containing 0.09 % Sodium Azide / PEG and Sucrose.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution,aliquote and freeze in liquid nitrogen, reconstituted antibody can be stored frozen at-80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3months. Avoid repeated freeze / thaw cycles!
- Date de péremption
- 12 months
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- Antigène
- EGFR (Epidermal Growth Factor Receptor (EGFR))
- Autre désignation
- EGFR / ERBB1 (EGFR Produits)
- Synonymes
- anticorps C-erb, anticorps CG10079, anticorps D-EGFR, anticorps D-Egf, anticorps DEGFR, anticorps DER, anticorps DER flb, anticorps DER/EGFR, anticorps DER/faint little ball, anticorps DER/top, anticorps DER/torpedo, anticorps DER1, anticorps DEgfr, anticorps Degfr, anticorps Der, anticorps DmHD-33, anticorps Dmel\\CG10079, anticorps EFG-R, anticorps EGF-R, anticorps EGFR, anticorps EGFr, anticorps EGfr, anticorps EK2-6, anticorps Egf, anticorps Egf-r, anticorps EgfR, anticorps El, anticorps Elp, anticorps Elp-1, anticorps Elp-B1, anticorps Elp-B1RB1, anticorps HD-33, anticorps TOP, anticorps Torpedo/DER, anticorps Torpedo/Egfr, anticorps c-erbB, anticorps d-egf-r, anticorps dEGFR, anticorps dEGFR1, anticorps dEgfr, anticorps der, anticorps egfr, anticorps flb, anticorps l(2)05351, anticorps l(2)09261, anticorps l(2)57DEFa, anticorps l(2)57EFa, anticorps l(2)57Ea, anticorps mor1, anticorps top, anticorps top/DER, anticorps top/flb, anticorps torpedo/Egfr, anticorps torpedo/egfr, anticorps EGFR12, anticorps EGFR15, anticorps egfr1, anticorps Erbb2, anticorps ERBB, anticorps ERBB1, anticorps HER1, anticorps PIG61, anticorps mENA, anticorps ErbB-1, anticorps Errp, anticorps 9030024J15Rik, anticorps AI552599, anticorps Erbb, anticorps Errb1, anticorps Wa5, anticorps wa-2, anticorps wa2, anticorps epidermal growth factor receptor, anticorps Epidermal growth factor receptor, anticorps epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian), anticorps EGFR, anticorps Egfr, anticorps egfra, anticorps egfr1, anticorps LOC5564544
- Sujet
- EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1
- ID gène
- 1956
- UniProt
- P00533
- Pathways
- Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
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