MEK1 anticorps (pSer218, pSer222, pSer226)
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- Antigène Voir toutes MEK1 (MAP2K1) Anticorps
- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
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Épitope
- pSer218, pSer222, pSer226
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Reactivité
- Humain, Souris, Chien
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp MEK1 est non-conjugé
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificité
- AM00090PU-N specifically recognizes MEK1 phosphorylated at serine 218/222 and MEK2 phosphorylated at serine 222/226.
- Attributs du produit
- Synonyms: MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK1, PRKMK1, MAP kinase kinase 1,Dual specificity mitogen-activated protein kinase kinase 1
- Purification
- Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
- Ingrédients
- incl. positive Control
- Immunogène
- Phosphopeptide conjugated to KLH. Epitope: pSer 218/222 in MEK1 pSer 222/226 in MEK2AA Sequence: D pS M A N pS FRemarks: Mol. Weight of Antigen: 45 kDa
- Clone
- 7-00E-010
- Isotype
- IgG1
- Top Product
- Discover our top product MAP2K1 Anticorps primaire
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- Indications d'application
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Suitable for Western Blot (0.5 μg/mL for HRPO/ECL detection)Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use SW480 pervanadate-treated as positive control (see Protocols below). ELISA: 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocole
- Contains Positive Control (pervanadate treated SW 480 cells)Format: Lyophilized cell lysate from SW480 cells. Serum starved cells were treated for15min with pervanadate. Reconstitution: Add 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGEsample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRP/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation. Aliquot and store frozen. Avoid repeated freeze/thaw cycles.
- Restrictions
- For Research Use only
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- Reconstitution
- Reconstitute with 1 mL H2O (15 min, RT)
- Buffer
- 1 x PBS / 0.09 % Na-azide / PEG and Sucrose
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
- Date de péremption
- 12 months
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- Antigène
- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
- Autre désignation
- MAP2K1 (MAP2K1 Produits)
- Synonymes
- anticorps Mek1, anticorps CFC3, anticorps MAPKK1, anticorps MEK1, anticorps MKK1, anticorps PRKMK1, anticorps MEKK1, anticorps Prkmk1, anticorps mek-2, anticorps si:ch211-242m18.2, anticorps wu:fj56a12, anticorps wu:fj61b01, anticorps zgc:56557, anticorps ATMEK1, anticorps F20B18.180, anticorps F20B18_180, anticorps MAP kinase/ ERK kinase 1, anticorps MITOGEN ACTIVATED PROTEIN KINASE KINASE, anticorps MITOGEN ACTIVATED PROTEIN KINASE KINASE 1, anticorps NMAPKK, anticorps MEK, anticorps mitogen activated protein kinase kinase 1, anticorps mitogen-activated protein kinase kinase 1, anticorps mitogen-activated protein kinase kinase 1 L homeolog, anticorps MAP kinase/ ERK kinase 1, anticorps Map2k1, anticorps MAP2K1, anticorps map2k1.L, anticorps map2k1, anticorps MEK1
- Sujet
- MEK (MAP Kinase Kinase) phosphorylates the MAP Kinase 1 and 2 on both threonine and tyrosine residues of the activation loop motif TEY. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser 218/222 in MEK1 and Ser 222/226 in MEK2). These phosphorylation sites are substrates of the Raf family of kinases.Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1
- ID gène
- 5604
- UniProt
- Q02750
- Pathways
- Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
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