AKT1 anticorps (C-Term, Ser473)
-
- Antigène Voir toutes AKT1 Anticorps
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
-
Épitope
- C-Term, Ser473
-
Reactivité
- Humain, Souris, Rat, Chien
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp AKT1 est non-conjugé
-
Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificité
- 11A11 specifically recognizes PKB when it is dephosphorylated at serine 473.
- Attributs du produit
- Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase
- Purification
- Subsequent thiophilic adsorption and size exclusion chromatography
- Ingrédients
- incl. positive Control
- Immunogène
- Synthetic peptide conjugated to KLH. Epitope: C-terminus. Remarks: Molecular weight of antigen: 60 kDa
- Clone
- 11A11
- Isotype
- IgG1
- Top Product
- Discover our top product AKT1 Anticorps primaire
-
-
- Indications d'application
-
Suitable for Western blot: 0.5 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use cell lysate from untreated HepG2 cells (provided with this antibody). ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocole
- POSITIVE CONTROL CELL LYSATE: HepG2 untreatedFormat:
- Restrictions
- For Research Use only
-
- Reconstitution
- Restore with 1 mL H2O (15 min, RT).
- Stock
- -20 °C
- Stockage commentaire
- For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution,aliquote and freeze in liquid nitrogen, reconstituted antibody can be stored frozen at -80 °Cup to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freeze / thaw cycles.
- Date de péremption
- 12 months
-
- Antigène
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
- Autre désignation
- AKT1 / PKB (AKT1 Produits)
- Synonymes
- anticorps AKT, anticorps CWS6, anticorps PKB, anticorps PKB-ALPHA, anticorps PRKBA, anticorps RAC, anticorps RAC-ALPHA, anticorps Akt, anticorps Rac, anticorps PKB/Akt, anticorps PKBalpha, anticorps AKT/PKB, anticorps AKT1, anticorps Akt/PKB, anticorps Akt1, anticorps CG4006, anticorps D-Akt, anticorps DAKT1, anticorps DAKT1/PKB, anticorps DAkt, anticorps DAkt1, anticorps DPKB, anticorps DRAC-PK, anticorps DRAC-PK66, anticorps DRAC-PK85, anticorps Dakt, anticorps Dakt1, anticorps Dmel\\CG4006, anticorps Dpkb, anticorps PKB/AKT, anticorps PKB/dAKT, anticorps RacPK, anticorps akt, anticorps akt1, anticorps dAKT, anticorps dAKT/dPKB, anticorps dAKT1, anticorps dAkt, anticorps dAkt/PKB, anticorps dAkt1, anticorps dPKB, anticorps dakt, anticorps dakt1, anticorps l(3)04226, anticorps l(3)89Bq, anticorps p-Akt, anticorps pAkt, anticorps ACT-5, anticorps akt-1, anticorps pkb, anticorps v-akt, anticorps v-akt1, anticorps xAct, anticorps ATAKT1, anticorps F18A8.2, anticorps F18A8_2, anticorps K+ transporter 1, anticorps POTASSIUM TRANSPORTER, anticorps AKT serine/threonine kinase 1, anticorps thymoma viral proto-oncogene 1, anticorps CG4006 gene product from transcript CG4006-RE, anticorps actin beta, anticorps v-akt murine thymoma viral oncogene homolog 1 S homeolog, anticorps v-akt murine thymoma viral oncogene homolog 1, anticorps K+ transporter 1, anticorps Serine/threonine-protein kinase akt-1, anticorps AKT1, anticorps Akt1, anticorps ACTB, anticorps akt1.S, anticorps akt1, anticorps KT1, anticorps akt-1
- Sujet
- The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far, the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
- ID gène
- 207
- UniProt
- P31749
- Pathways
- Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, L'effet Warburg
-