AKT2 anticorps
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- Antigène Voir toutes AKT2 Anticorps
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp AKT2 est non-conjugé
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificité
- This antibody specifically recognizes the beta isoform of PKB (PKBβ/Akt2).
- Attributs du produit
- Synonyms: RAC-PK-beta, Protein kinase Akt-2, Protein kinase B beta, RAC-betaserine/threonine-protein kinase
- Purification
- Thiophilic Adsorption and Size Exclusion Chromatography.
- Ingrédients
- incl. positive Control
- Immunogène
- Synthetic peptide conjugated to KLH
- Clone
- 8B7
- Isotype
- IgG2a
- Top Product
- Discover our top product AKT2 Anticorps primaire
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- Indications d'application
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Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1 mL H2O (15 min, RT).
- Buffer
- 1 mL, 2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C/-80 °C
- Stockage commentaire
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. - Date de péremption
- 12 months
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- Antigène
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
- Autre désignation
- AKT2 / PKB beta (AKT2 Produits)
- Synonymes
- anticorps AKT2, anticorps pkbb, anticorps pkbbeta, anticorps pkbss, anticorps prkbb, anticorps rac-beta, anticorps v-akt2, anticorps 2410016A19Rik, anticorps AW554154, anticorps PKB, anticorps PKBbeta, anticorps HIHGHH, anticorps PKBB, anticorps PKBBETA, anticorps PRKBB, anticorps RAC-BETA, anticorps cb945, anticorps akt2-a, anticorps AKT serine/threonine kinase 2, anticorps v-akt murine thymoma viral oncogene homolog 2, anticorps thymoma viral proto-oncogene 2, anticorps v-akt murine thymoma viral oncogene homolog 2 L homeolog, anticorps AKT2, anticorps akt2, anticorps Akt2, anticorps akt2.L
- Sujet
- Akt, protein kinase B (PKB), is a serine/threonine kinase, which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers. AKT2 encodes a RAC/AKT-type protein kinase that contains a N-terminal pleckstrin-homology (PH) domain and a central catalytic domain closely related to both cAMP-dependent protein kinase and protein kinase C. The protein is a member of PI3K-mediated signalling pathways associated with the regulation of proliferation, survival, protein synthesis, and metabolism. It is activated by a variety of growth factors. AKT2 has been shown to be transcriptionally regulated by MyoD and to activate MyoD-myocyte enhancer binding factor-2 (MEF2) transactivation activity during muscle differentiation. Glycogen synthase kinase 3 (GSK-3) also has been shown to be a downstream target of AKT2. The AKT2 gene is one of the human homologues of v-akt, the transduced oncogene of the AKT8 virus, which induces lymphomas in mice. It has been implicated in breast, ovarian, and pancreatic cancers.Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase
- ID gène
- 208
- UniProt
- P31751
- Pathways
- Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Signalisation TLR, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
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