Western Blotting (WB), Immunoprecipitation (IP), Fluorescence Microscopy (FM)
Attributs du produit
Concentration Definition: by UV absorbance at 280 nm
Immunogène
This protein A purified monoclonal antibody was produced by repeated immunizations with full-length recombinant human MAD2L1 protein. Immunogentype:Recombinant
MAD2L1
Reactivité: Humain
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
This protein A purified antibody has been tested for use in immunoprecipitation and by western blot. This antibody is not applicable for immunofluorescence microscopy. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 24 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.
Restrictions
For Research Use only
Format
Liquid
Concentration
1.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
MAD2L1 (also called mitotic spindle assembly checkpoint protein, MAD2A, MAD2-like 1 and HsMAD2) is a component of the mitotic spindle assembly checkpoint monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. MAD2L1 inhibits the activity of the anaphase-promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. This protein has a nuclear localization. A MAD2 pseudogene has been mapped to chromosome 14 Synonyms: Mitotic arrest deficient yeast homolog like 1 antibody, Mitotic spindle assembly checkpoint protein antibody