PRKDC anticorps (pThr2609)

N° du produit ABIN129603

Détail du produit anti-PRKDC anticorps

Antigène: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Épitope: pThr2609

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRKDC est non-conjugé

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Réactivité croisée: Chimpanzé

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Thr 2609 of human DNA PKcs.

Isotype: IgG

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 460 kDa in size corresponding to DNA PKcs by western blotting in the appropriate cell lysate or extract.   Alternate splice variants have been described for this protein.  Best western blotting results are seen when IP is performed prior to detection.  This antibody detects an inducible signal at the correct height after DNA damage.  However, the antibody may also detect an IR-inducible signal in cells lacking DNA PKcs (MO59J cells) at the same size as DNA PKcs if western blotting is performed directly.  We believe that this additional band is 53BP1 that runs at the same size as DNA PKcs and is also phosphorylated in an IR-dependent manner. Similar results were seen with another phospho DNA PKcs antibody indicating that this result is general to antibodies to this phospho site, rather than specific to this antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.93 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-PRKDC (ABIN129603)

Yoshikawa, Kashino, Ono, Watanabe: "Phosphorylated H2AX foci in tumor cells have no correlation with their radiation sensitivities." dans: Journal of radiation research, Vol. 50, Issue 2, pp. 151-60, (2009) (PubMed).

Wanner, Mayer, Kehlbach, Rodemann, Dittmann: "Activation of protein kinase Cepsilon stimulates DNA-repair via epidermal growth factor receptor nuclear accumulation." dans: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, Vol. 86, Issue 3, pp. 383-90, (2008) (PubMed).

Dittmann, Mayer, Kehlbach, Rodemann: "The radioprotector Bowman-Birk proteinase inhibitor stimulates DNA repair via epidermal growth factor receptor phosphorylation and nuclear transport." dans: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, Vol. 86, Issue 3, pp. 375-82, (2008) (PubMed).

Dittmann, Mayer, Wanner, Kehlbach, Rodemann: "The radioprotector O-phospho-tyrosine stimulates DNA-repair via epidermal growth factor receptor- and DNA-dependent kinase phosphorylation." dans: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, Vol. 84, Issue 3, pp. 328-34, (2007) (PubMed).

Dittmann, Mayer, Ohneseit, Raju, Andratschke, Milas, Rodemann: "Celecoxib induced tumor cell radiosensitization by inhibiting radiation induced nuclear EGFR transport and DNA-repair: a COX-2 independent mechanism." dans: International journal of radiation oncology, biology, physics, Vol. 70, Issue 1, pp. 203-12, (2007) (PubMed).

Détails sur PRKDC

Antigène: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Autre désignation: DNA PKcs (PRKDC Produits)

Synonymes: anticorps DNA-PKcs, anticorps DNAPK, anticorps DNPK1, anticorps HYRC, anticorps HYRC1, anticorps XRCC7, anticorps p350, anticorps DNA-PKCS, anticorps dna-pkcs, anticorps dnapk, anticorps dnpk1, anticorps hyrc, anticorps hyrc1, anticorps prkdc-A, anticorps xrcc7, anticorps PRKDC, anticorps AI326420, anticorps AU019811, anticorps DNAPDcs, anticorps DOXNPH, anticorps dxnph, anticorps p460, anticorps scid, anticorps slip, anticorps protein kinase, DNA-activated, catalytic polypeptide, anticorps protein kinase, DNA-activated, catalytic polypeptide L homeolog, anticorps DNA-dependent protein kinase catalytic subunit, anticorps protein kinase, DNA activated, catalytic polypeptide, anticorps PRKDC, anticorps prkdc.L, anticorps LOC100633457, anticorps Prkdc

Sujet: DNA dependent Protein Kinase (also called DNAPK, DNPK1, HYRC1, Protein Kinase DNA Activated Catalytic Polypeptide, XRCC7 and p460) consists of the 460 kDa DNA PKcs and a heterodimeric regulatory complex comprised of p70 Ku and p80 Ku (Ku autoantigen). DNA PKcs is a nuclear protein serine/threonine kinase present in a wide variety of eukaryotic species. DNA PKcs phosphorylates transcription factors, Sp1, Oct-1, p53 and SV40 large T antigen. DNA PKcs is involved in repairing double stranded DNA breaks. At the onset of apoptosis, DNA PKcs is rapidly inactivated by cleavage of the catalytic subunit into smaller polypeptides. Proteolysis of DNA PKcs is inhibited by the cysteine protease inhibitors iodoacetamide and N-ethylmaleimide.  Alternative splicing can occur for this protein to produce at least two isoforms.
Synonyms: DNA dependent protein kinase catalytic subunit antibody, DNA PK catalytic subunit antibody, DNAPK antibody, DNAPK catalytic subunit antibody, DNPK 1 antibody, DNPK1 antibody, HYRC 1 antibody

ID gène: 5591, 13654237

UniProt: P78527

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response