Aurora Kinase B anticorps (pThr232)

N° du produit ABIN129612

Détail du produit anti-Aurora Kinase B anticorps

Antigène: Aurora Kinase B (AURKB)

Épitope: AA 223-234, pThr232

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Aurora Kinase B est non-conjugé

Application: Western Blotting (WB), ELISA

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 223-234 of Human Aurora Kinase B protein.

Isotype: IgG

Information d'application

Indications d'application: This affinity-purified antibody has been tested for use in ELISA and by western blot.  See below for specific protocol.  Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract.  HeLa cell lysate can be used as a positive control.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.21 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-Aurora Kinase B (ABIN129612)

Barroso-Vilares, Macedo, Reis, Warren, Compton, Logarinho: "Small-molecule inhibition of aging-associated chromosomal instability delays cellular senescence." dans: EMBO reports, Vol. 21, Issue 5, pp. e49248, (2020) (PubMed).

Achuthankutty, Thakur, Haahr, Hoffmann, Drainas, Bizard, Weischenfeldt, Hickson, Mailand: "Regulation of ETAA1-mediated ATR activation couples DNA replication fidelity and genome stability." dans: The Journal of cell biology, Vol. 218, Issue 12, pp. 3943-3953, (2020) (PubMed).

Novais-Cruz, Alba Abad, van IJcken, Galjart, Jeyaprakash, Maiato, Ferrás: "Mitotic progression, arrest, exit or death relies on centromere structural integrity, rather than de novo transcription." dans: eLife, Vol. 7, (2019) (PubMed).

Liu, Kwon, Mannino, Yang, Renda, Khodjakov, Pellman: "Nuclear envelope assembly defects link mitotic errors to chromothripsis." dans: Nature, Vol. 561, Issue 7724, pp. 551-555, (2019) (PubMed).

Xie, Lin, Wu, Tan, Cheng, Zhang: "Cardiac glycoside bufalin blocks cancer cell growth by inhibition of Aurora A and Aurora B activation via PI3K-Akt pathway." dans: Oncotarget, Vol. 9, Issue 17, pp. 13783-13795, (2018) (PubMed).

Skowyra, Allan, Saurin, Clarke: "USP9X Limits Mitotic Checkpoint Complex Turnover to Strengthen the Spindle Assembly Checkpoint and Guard against Chromosomal Instability." dans: Cell reports, Vol. 23, Issue 3, pp. 852-865, (2018) (PubMed).

Drpic, Almeida, Aguiar, Renda, Damas, Lewin, Larkin, Khodjakov, Maiato: "Chromosome Segregation Is Biased by Kinetochore Size." dans: Current biology : CB, Vol. 28, Issue 9, pp. 1344-1356.e5, (2018) (PubMed).

de Cárcer, Venkateswaran, Salgueiro, El Bakkali, Somogyi, Rowald, Montañés, Sanclemente, Escobar, de Martino, McGranahan, Malumbres, Sotillo: "Plk1 overexpression induces chromosomal instability and suppresses tumor development." dans: Nature communications, Vol. 9, Issue 1, pp. 3012, (2018) (PubMed).

Shrestha, Conti, Tamura, Braun, Ramalingam, Cieslinski, Ries, Draviam: "Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells." dans: Nature communications, Vol. 8, Issue 1, pp. 150, (2017) (PubMed).

Mo, Zhuang, Liu, Yao, Qin, Su, Zang, Wang, Zhang, Dou, Tian, Teng, Niu, Hill, Fang, Ding, Fu, Yao: "Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation." dans: Nature chemical biology, Vol. 12, Issue 4, pp. 226-32, (2016) (PubMed).

Redli, Gasic, Meraldi, Nigg, Santamaria: "The Ska complex promotes Aurora B activity to ensure chromosome biorientation." dans: The Journal of cell biology, Vol. 215, Issue 1, pp. 77-93, (2016) (PubMed).

Asghar, Lajeunesse, Dulla, Combes, Thebault, Nigg, Elowe: "Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation." dans: Nature communications, Vol. 6, pp. 8364, (2015) (PubMed).

Ohishi, Muramatsu, Yoshida, Seimiya: "TRF1 ensures the centromeric function of Aurora-B and proper chromosome segregation." dans: Molecular and cellular biology, Vol. 34, Issue 13, pp. 2464-78, (2014) (PubMed).

Carretero, Ruiz-Torres, Rodríguez-Corsino, Barthelemy, Losada: "Pds5B is required for cohesion establishment and Aurora B accumulation at centromeres." dans: The EMBO journal, Vol. 32, Issue 22, pp. 2938-49, (2014) (PubMed).

Nijenhuis, Vallardi, Teixeira, Kops, Saurin: "Negative feedback at kinetochores underlies a responsive spindle checkpoint signal." dans: Nature cell biology, Vol. 16, Issue 12, pp. 1257-64, (2014) (PubMed).

Rivera, Ghenoiu, Rodríguez-Corsino, Mochida, Funabiki, Losada: "Xenopus Shugoshin 2 regulates the spindle assembly pathway mediated by the chromosomal passenger complex." dans: The EMBO journal, Vol. 31, Issue 6, pp. 1467-79, (2012) (PubMed).

Mackay, Makise, Ullman: "Defects in nuclear pore assembly lead to activation of an Aurora B-mediated abscission checkpoint." dans: The Journal of cell biology, Vol. 191, Issue 5, pp. 923-31, (2011) (PubMed).

Wheatley, Henzing, Dodson, Khaled, Earnshaw: "Aurora-B phosphorylation in vitro identifies a residue of survivin that is essential for its localization and binding to inner centromere protein (INCENP) in vivo." dans: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5655-60, (2004) (PubMed).

Détails sur Aurora Kinase B

Antigène: Aurora Kinase B (AURKB)

Autre désignation: Aurora B (AURKB Produits)

Synonymes: anticorps AIK2, anticorps AIM-1, anticorps AIM1, anticorps ARK2, anticorps AurB, anticorps IPL1, anticorps PPP1R48, anticorps STK12, anticorps STK5, anticorps aurkb-sv1, anticorps aurkb-sv2, anticorps AIRK2, anticorps AL022959, anticorps Aik2, anticorps Aim1, anticorps Ark2, anticorps STK-1, anticorps Stk12, anticorps Stk5, anticorps Aur B, anticorps Aurora B, anticorps Aurora-B, anticorps CG6620, anticorps DmAIRK2, anticorps DmAurB, anticorps DmAuroraB, anticorps Dmel\\CG6620, anticorps IAL, anticorps IAL1, anticorps Ial, anticorps aurB, anticorps aurora B, anticorps ARK-2, anticorps XAIRK2, anticorps aik2, anticorps aim-1, anticorps aim1, anticorps airk2, anticorps ark2, anticorps ipl1, anticorps stk12, anticorps stk5, anticorps CHUNP6896, anticorps fa09g06, anticorps stka, anticorps wu:fa09g06, anticorps aurkb, anticorps AIRK2-B, anticorps XAIRK2-B, anticorps aurkb-a, anticorps aurkb-b, anticorps xAurora-B-B, anticorps aurora kinase B, anticorps aurora B, anticorps aurora kinase B S homeolog, anticorps AURKB, anticorps Aurkb, anticorps aurB, anticorps aurkb, anticorps aurkb.S

Sujet: Aurora Kinase B (also known as Aurora and Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, STK-1, and Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body.  High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
Synonyms: AIK2 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, AURKB antibody, Aurora 1 antibody, Aurora and Ipl1 like midbody associated protein 1 antibody

ID gène: 9212, 83776600

UniProt: Q96GD4

Pathways: TCR Signaling, Cycle Cellulaire, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades