RAD52 anticorps (AA 36-0)

N° du produit ABIN129613

Détail du produit anti-RAD52 anticorps

Antigène: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Épitope: AA 36-0

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAD52 est non-conjugé

Application: Western Blotting (WB), ELISA

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 360 - 375 of the Human Rad 52 protein.

Isotype: IgG

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in western blotting using HeLa nuclear extracts.  The antibody is also functional by ELISA.  Dilutions for western blotting represent a starting point dilution and further optimization may be required.  The antibody detects a band of approximately 63.0 kDa (predicted molecular weight: 46.3 kDa). Specific band detection by western blot is blocked by peptide competition by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane.  Reactivity in other immunoassays is unknown.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détails sur RAD52

Antigène: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Autre désignation: RAD52 (RAD52 Produits)

Synonymes: anticorps Rad52yh, anticorps RAD52, anticorps zgc:112400, anticorps LOC100223085, anticorps MGC115453, anticorps RAD52 homolog, DNA repair protein, anticorps RAD52 homolog (S. cerevisiae), anticorps RAD52 homolog, DNA repair protein S homeolog, anticorps recombinase, anticorps DNA repair and recombination protein, putative, anticorps RAD52 protein, anticorps RAD52, anticorps Rad52, anticorps rad52, anticorps rad52.S

Sujet: The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair.   Four alternatively spliced transcript variants encoding different isoforms have been reported for this gene.  The alpha variant encodes the longest isoform that contains an identical N-terminus, but a distinct C-terminus, as compared to other isoforms (beta, delta, and gamma).
Synonyms: DNA repair protein RAD52 homolog antibody, RAD 52 antibody, RAD52 homolog (S. cerevisiae) antibody, RAD52 homolog antibody, Recombination protein RAD52 antibody, Rhabdomyosarcoma antigen MU RMS 40.23 antibody

ID gène: 5893

NCBI Accession: NP_602296

UniProt: P43351

Pathways: Réparation de l'ADN