GLI3 anticorps (AA 41-57)

N° du produit ABIN129623

Détail du produit anti-GLI3 anticorps

Antigène: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Épitope: AA 41-57

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLI3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Réactivité croisée: Chimpanzé, Squirrel Monkey (Saimiri spec.), Xenopus laevis, Poulet, Chien, Caille

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: This affinity purified antibody was produced from monospecific rabbit serum by repeated immunizations with a synthetic peptide corresponding to amino acids 41-57 of human Gli-3 protein.

Isotype: IgG

Information d'application

Indications d'application: This antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Detection of Gli-3 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-3 is likely to be low. Furthermore, Gli3 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-GLI3 (ABIN129623)

Hehemann, Kalmanek, Choe, Dynda, Hu, Quek, Harrington, Stupp, McVary, Podlasek: "Sonic hedgehog regulation of human rhabdosphincter muscle:Potential implications for treatment of stress urinary incontinence." dans: Neurourology and urodynamics, Vol. 37, Issue 8, pp. 2551-2559, (2019) (PubMed).

Détails sur GLI3

Antigène: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Autre désignation: Gli-3 (GLI3 Produits)

Synonymes: anticorps ACLS, anticorps GCPS, anticorps GLI3-190, anticorps GLI3FL, anticorps PAP-A, anticorps PAPA, anticorps PAPA1, anticorps PAPB, anticorps PHS, anticorps PPDIV, anticorps xgli3, anticorps AI854843, anticorps AU023367, anticorps Bph, anticorps Pdn, anticorps Xt, anticorps add, anticorps im:7142603, anticorps GLI3, anticorps GLI family zinc finger 3, anticorps GLI family zinc finger 3 L homeolog, anticorps GLI-Kruppel family member GLI3, anticorps GLI3, anticorps Gli3, anticorps gli3.L, anticorps gli3

Sujet: Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS); an autosomal dominant disorder-affecting limb and cranio-facial development.  Two isoforms of human Gli-3 have been reported.  One is the full-length protein at ~170-190kDa and the other is a truncated isoform at ~80kDa.
Synonyms: Gli-3, Zinc Finger Protein Gli-3, GLI-Kruppel family member GLI-3

ID gène: 2737, 119393899

UniProt: P10071

Pathways: Signalisation Hedgehog