beta Actin anticorps (AA 359-368)

N° du produit ABIN129657

Détail du produit anti-beta Actin anticorps

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Épitope: AA 359-368

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp beta Actin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Réactivité croisée: Rat (Rattus), Humain, Singe, Mouton, Boeuf (Vache), Chévre, Cobaye, Porc, Hamster

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 359-368 of Human beta Actin.

Isotype: IgG

Informations sur le produit:

À quoi peut servir l'anticorps bêta-Actin ABIN129657 ? Cet anticorps polyclonal non conjugué de la bêta-Actine de lapin convient à la détection de la bêta-Actine (ACTB) par immunohistochimie, ELISA et Western blotting. L'anticorps reconnaît la bêta-actine humaine.

Quelles sont les données de validation disponibles pour cet anticorps anti-bêta-actine ? L'anticorps a été utilisé dans 31 publications à ce jour, qui sont énumérées ci-dessous et peuvent être consultées sur Pubmed. Il y a 10 images de produits disponibles montrant la performance de l'anticorps beta-Actin dans les western blots. Utilisez notre anticorps de haute qualité pour détecter de manière fiable la bêta-Actine.

Quelle est la fonction de l'actine ? L'actine est une protéine hautement conservée qui se polymérise pour produire des filaments qui forment des réseaux réticulés dans le cytoplasme des cellules. L'actine existe à la fois sous forme monomérique (G-actine) et polymérique (F-actine), les deux jouant des fonctions clés, comme la motilité et la contraction des cellules. En plus de leur rôle dans le cytosquelette cytoplasmique, la G- et la F-actine se localisent également dans le noyau et régulent la transcription et la motilité des gènes ainsi que la réparation de l'ADN endommagé. (UniProt)

Information d'application

Indications d'application: Anti-beta Actin Antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-beta Actin (ABIN129657)

Olive, Qin, DeCristo, Laszewski, Greathouse, McAllister: "Accounting for tumor heterogeneity when using CRISPR-Cas9 for cancer progression and drug sensitivity studies." dans: PLoS ONE, Vol. 13, Issue 6, pp. e0198790, (2019) (PubMed).

Dai, Luftig: "Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 200, Issue 5, pp. 1727-1736, (2019) (PubMed).

Borroni, Emanuelli, Shah, Ilić, Apel-Sarid, Paolini, Manikoth Ayyathan, Koganti, Levy-Cohen, Blank: "Smurf2 regulates stability and the autophagic-lysosomal turnover of lamin A and its disease-associated form progerin." dans: Aging cell, Vol. 17, Issue 2, (2018) (PubMed).

Coleman, Ha, Haeryfar, Barr, Kim: "Cathepsin B plays a key role in optimal production of the influenza A virus." dans: Journal of virology & antiviral research, Vol. 2018, pp. 1-20, (2018) (PubMed).

Lodola, Laforenza, Cattaneo, Ruffinatti, Poletto, Massa, Tancredi, Zuccolo, Khdar, Riccardi, Biggiogera, Rosti, Guerra, Moccia: "VEGF-induced intracellular Ca2+ oscillations are down-regulated and do not stimulate angiogenesis in breast cancer-derived endothelial colony forming cells." dans: Oncotarget, Vol. 8, Issue 56, pp. 95223-95246, (2017) (PubMed).

Chen, Wang, Man, Li, Shan: "Increased B7-H4 expression during esophageal squamous cell carcinogenesis is associated with IL-6/STAT3 signaling pathway activation in mice." dans: Oncology letters, Vol. 13, Issue 4, pp. 2207-2215, (2017) (PubMed).

Rae, Hocum, Bii, Deeg, Trobridge: "A novel retroviral mutagenesis screen identifies prognostic genes in RUNX1 mediated myeloid leukemogenesis." dans: Oncotarget, Vol. 6, Issue 31, pp. 30664-74, (2016) (PubMed).

Koh, Powell, Blake, Hoffman, Hopkins, Feng: "Enhanced cytotoxicity in triple-negative and estrogen receptor‑positive breast adenocarcinoma cells due to inhibition of the transient receptor potential melastatin-2 channel." dans: Oncology reports, Vol. 34, Issue 3, pp. 1589-98, (2016) (PubMed).

Campos, Qiu, Gomero, Wakefield, Horner, Brutkowski, Han, Solecki, Frase, Bongiovanni, dAzzo: "Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier." dans: Nature communications, Vol. 7, pp. 11876, (2016) (PubMed).

Hopkins, Feng, Liu, Parker, Koh: "Inhibition of the transient receptor potential melastatin-2 channel causes increased DNA damage and decreased proliferation in breast adenocarcinoma cells." dans: International journal of oncology, Vol. 46, Issue 5, pp. 2267-76, (2015) (PubMed).

Maragkoudaki, Vaiopoulou, Theodoropoulos, Legaki, Sechi, Karamanolis, Zografos, Gazouli: "Specific detection of OCT4 isoforms in inflammatory bowel disease." dans: Gut pathogens, Vol. 7, pp. 25, (2015) (PubMed).

Zha, Xia, Ding, Choi, Yang, Dong, Yan, Huang, Ding: "MEIS2 is essential for neuroblastoma cell survival and proliferation by transcriptional control of M-phase progression." dans: Cell death & disease, Vol. 5, pp. e1417, (2015) (PubMed).

Nelson, Estus, Abner, Parikh, Malik, Neltner, Ighodaro, Wang, Wilfred, Wang, Kukull, Nandakumar, Farman, Poon, Corrada, Kawas, Cribbs, Bennett, Schneider, Larson, Crane, Valladares, Schmitt, Kryscio et al.: "ABCC9 gene polymorphism is associated with hippocampal sclerosis of aging pathology. ..." dans: Acta neuropathologica, Vol. 127, Issue 6, pp. 825-43, (2015) (PubMed).

Wang, Choi, Ding, Yang, Ngoka, Lee, Zha, Mao, Jin, Ren, Cowell, Huang, Shi, Cui, Ding: "HOXC9 directly regulates distinct sets of genes to coordinate diverse cellular processes during neuronal differentiation." dans: BMC genomics, Vol. 14, pp. 830, (2014) (PubMed).

Dragoni, Laforenza, Bonetti, Reforgiato, Poletto, Lodola, Bottino, Guido, Rappa, Pareek, Tomasello, Guarrera, Cinelli, Aronica, Guerra, Barosi, Tanzi, Rosti, Moccia: "Enhanced expression of Stim, Orai, and TRPC transcripts and proteins in endothelial progenitor cells isolated from patients with primary myelofibrosis." dans: PLoS ONE, Vol. 9, Issue 3, pp. e91099, (2014) (PubMed).

Dragoni, Turin, Laforenza, Potenza, Bottino, Glasnov, Prestia, Ferulli, Saitta, Mosca, Guerra, Rosti, Luinetti, Ganini, Porta, Pedrazzoli, Tanzi, Montagna, Moccia: "Store-operated Ca2+ entry does not control proliferation in primary cultures of human metastatic renal cellular carcinoma." dans: BioMed research international, Vol. 2014, pp. 739494, (2014) (PubMed).

Wang, Danaher, Miller, Berger, Nubia, Wilfred, Neltner, Norris, Nelson: "Expression of miR-15/107 family microRNAs in human tissues and cultured rat brain cells." dans: Genomics, proteomics & bioinformatics, Vol. 12, Issue 1, pp. 19-30, (2014) (PubMed).

Papaconstantinou, Zeglinas, Gazouli, Nastos, Yiallourou, Lykoudis, Evangelou, Papalois, Papaioannou, Vlachogiannakos, Tzathas: "Effect of infliximab on the healing of intestinal anastomosis. An experimental study in rats." dans: International journal of surgery (London, England), Vol. 12, Issue 9, pp. 969-75, (2014) (PubMed).

Laforenza, Scaffino, Gastaldi: "Aquaporin-10 represents an alternative pathway for glycerol efflux from human adipocytes." dans: PLoS ONE, Vol. 8, Issue 1, pp. e54474, (2013) (PubMed).

Lodola, Laforenza, Bonetti, Lim, Dragoni, Bottino, Ong, Guerra, Ganini, Massa, Manzoni, Ambudkar, Genazzani, Rosti, Pedrazzoli, Tanzi, Moccia, Porta: "Store-operated Ca2+ entry is remodelled and controls in vitro angiogenesis in endothelial progenitor cells isolated from tumoral patients." dans: PLoS ONE, Vol. 7, Issue 9, pp. e42541, (2013) (PubMed).

Détails sur beta Actin

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Autre désignation: beta Actin (ACTB Produits)

Synonymes: anticorps BRWS1, anticorps PS1TP5BP1, anticorps ACTA1, anticorps Actx, anticorps E430023M04Rik, anticorps beta-actin, anticorps ps1tp5bp1, anticorps act-2, anticorps actb, anticorps B-actin, anticorps LOC100174883, anticorps LOC100304816, anticorps A, anticorps ACT5C, anticorps Ac5C, anticorps Act, anticorps Act-5C, anticorps Act5, anticorps Act5c, anticorps Actin, anticorps Actin/BAP47, anticorps Actin5C, anticorps BAP47, anticorps Bap47, anticorps CG4027, anticorps Dmel\\CG4027, anticorps M32055, anticorps T11, anticorps act, anticorps act 5C, anticorps act5C, anticorps actin, anticorps actin5C, anticorps anon-EST:fe2D2, anticorps beta-actin/Bap47, anticorps cyt5C, anticorps l(1)G0009, anticorps l(1)G0010, anticorps l(1)G0025, anticorps l(1)G0079, anticorps l(1)G0117, anticorps l(1)G0177, anticorps l(1)G0245, anticorps l(1)G0330, anticorps l(1)G0420, anticorps l(1)G0486, anticorps Bact, anticorps ACT-5, anticorps ACTB, anticorps B-ACTZF, anticorps actba, anticorps bact, anticorps bactin1, anticorps bactzf, anticorps wu:fd18f05, anticorps A26C1B, anticorps POTE2alpha, anticorps POTEACTIN, anticorps Beta-actin, anticorps actin beta, anticorps actin, beta, anticorps cytoplasmic actin OlCA1, anticorps beta-actin, anticorps Actin 5C, anticorps actin gamma 1, anticorps actin, beta 1, anticorps actin, beta L homeolog, anticorps beta actin, anticorps POTE ankyrin domain family member F, anticorps actin, beta like 2, anticorps actin, cytoplasmic 1, anticorps ACTB, anticorps Actb, anticorps actb, anticorps LOC100174883, anticorps LOC100304816, anticorps Act5C, anticorps ACTG1, anticorps actb1, anticorps actb.L, anticorps LOC100136352, anticorps POTEF, anticorps ACTBL2, anticorps LOC109073280

Sujet: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta-Actin Loading Control Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
Synonyms: Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

ID gène: 4501885

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction