MLF1 anticorps (Thr78)

N° du produit ABIN129679

Détail du produit anti-MLF1 anticorps

Antigène: MLF1 (Myeloid Leukemia Factor 1 (MLF1))

Épitope: Thr78

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MLF1 est non-conjugé

Application: Western Blotting (WB), ELISA

Réactivité croisée: Chien, Mouton, Chimpanzé

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding of human MLF1IP protein.

Isotype: IgG

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 65 kDa in size corresponding to MLF1IP protein by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.06 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détails sur MLF1

Antigène: MLF1 (Myeloid Leukemia Factor 1 (MLF1))

Autre désignation: MLF1 (MLF1 Produits)

Synonymes: anticorps MLF1, anticorps HLS7, anticorps myeloid leukemia factor 1, anticorps myeloid leukemia factor 1 L homeolog, anticorps MLF1, anticorps mlf1.L, anticorps mlf1, anticorps Mlf1

Sujet: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate.  Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition.  Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry.  Mutations of these sites stabilize MLF1IP and inhibit mitotic progression.  Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation.  Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block.  Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset.  MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation.  Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.
Synonyms: FLJ23468 antibody, HHV8 LNAIP1 antibody, ICEN24 antibody, Kaposi Sarcoma Herpesvirus latent nuclear antigen interacting protein 1 antibody

ID gène: 79682, 38016935

UniProt: Q71F23