Myosin anticorps (AA 12-27)

N° du produit ABIN129691

Détail du produit anti-Myosin anticorps

Antigène: Myosin

Épitope: AA 12-27

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Myosin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Réactivité croisée: Souris, Rat (Rattus)

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 12-27 of human myosin light chain protein.

Isotype: IgG

Information d'application

Indications d'application: This affinity-purified antibody was tested by ELISA and immunoblotting and was found to be reactive with both the unphosphorylated and mono-phosphorylated forms of the protein.   Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.71 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-Myosin (ABIN129691)

Deng, Bhaidani, Sutherland, MacDonald, Walsh et al.: "Rho-associated kinase and zipper-interacting protein kinase, but not myosin light chain kinase, are involved in the regulation of myosin phosphorylation in serum-stimulated human arterial smooth ..." dans: PLoS ONE, Vol. 14, Issue 12, pp. e0226406, (2020) (PubMed).

Lahey, Ronaghan, Shang, Dion, Désilets, Leduc, MacNaughton: "Signaling pathways induced by serine proteases to increase intestinal epithelial barrier function." dans: PLoS ONE, Vol. 12, Issue 7, pp. e0180259, (2017) (PubMed).

Logue, Cartagena-Rivera, Baird, Davidson, Chadwick, Waterman: "Erk regulation of actin capping and bundling by Eps8 promotes cortex tension and leader bleb-based migration." dans: eLife, Vol. 4, pp. e08314, (2016) (PubMed).

Détails sur Myosin

Antigène: Myosin

Abstract: Myosin Produits

Synonymes: anticorps CG3201, anticorps DmMLC_C, anticorps Dmel\\CG3201, anticorps MyoII, anticorps mlc-c, anticorps nmELC, anticorps MYOSIN, anticorps myosin 1, anticorps DFNA4, anticorps DFNA4A, anticorps MHC16, anticorps MYH17, anticorps NMHC II-C, anticorps NMHC-II-C, anticorps PNMHH, anticorps myosin, anticorps MYH1, anticorps MYH2, anticorps MYH3, anticorps MYH4, anticorps MYH8, anticorps adult, anticorps embryonic, anticorps 2400004E04Rik, anticorps II-C, anticorps NHMCII, anticorps DROMHC, anticorps Dm nmII, anticorps DmnmII, anticorps Dronm-MII, anticorps E(br), anticorps MHC, anticorps Mhc-c, anticorps Mhc-c[1], anticorps Myo, anticorps Myo II, anticorps NMM, anticorps Zip, anticorps anon-WO0140519.37, anticorps l(2)02957, anticorps nmMHC, anticorps nmy-2, anticorps zpr, anticorps DmelCG15792, anticorps CG15792, anticorps Myosin light chain cytoplasmic, anticorps hypothetical protein, anticorps myosin 1, anticorps myosin heavy chain 9, anticorps myosin heavy chain 14, anticorps myosin, heavy chain 1B, skeletal muscle, anticorps myosin, heavy polypeptide 14, anticorps zipper, anticorps Mlc-c, anticorps T22C1.6, anticorps ATM1, anticorps MYH9, anticorps MYH14, anticorps Myh14, anticorps MYH1B, anticorps zip

Sujet: Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail.  The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons.  Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction.   Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain.  The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells.  This sequence differs from that of the sarcomeric/
Synonyms: Myosin regulatory light polypeptide 9 Myosin regulatory light chain 9 Myosin regulatory light chain MRLC1 Myosin regulatory light chain 2, smooth muscle isoform Myosin RLC MLC-2C 20 kDa myosin light chain

ID gène: 98932, 38605043

UniProt: P24844