RFP anticorps (HRP)

N° du produit ABIN129725

Détail du produit anti-RFP anticorps (HRP)

Antigène: RFP (Red Fluorescent Protein (RFP))

Reactivité: Discosoma

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RFP est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Immunogène: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence derived from the mushroom polyp coral Discosoma.
Immunogentype:Recombinant

Isotype: IgG

Information d'application

Indications d'application: Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Gentamicin sulfate

Conseil sur la manipulation: Do NOT add Sodium Azide!

Stock: 4 °C

Références pour anticorps anti-RFP (HRP) (ABIN129725)

Bohlen, El-Nahal, Sommer: "Transduction of Craniofacial Motoneurons Following Intramuscular Injections of Canine Adenovirus Type-2 (CAV-2) in Rhesus Macaques." dans: Frontiers in neuroanatomy, Vol. 13, pp. 84, (2019) (PubMed).

Détails sur RFP

Antigène: RFP (Red Fluorescent Protein (RFP))

Autre désignation: RFP (RFP Produits)

Sujet: Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS).
Synonyms: HRP, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583