The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Functional application: Blocking of T cell proliferative responses. Flow cytometry: Recommended dilution: 2 μg/mL. Immunohistochemistry: Recommended dilution: 5-10 μg/mL.
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.4
Agent conservateur
Azide free
Conseil sur la manipulation
Do not freeze.
Stock
4 °C
Stockage commentaire
Store at 2-8°C. Do not freeze.
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Beers, Burich, Kleijmeer, Griffith, Wong, Rudensky: "Cathepsin S controls MHC class II-mediated antigen presentation by epithelial cells in vivo." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 3, pp. 1205-12, (2005) (PubMed).
Zang, Kalache, Lin, Schroppel, Murphy: "MHC Class II-mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C." dans: Journal of the American Society of Nephrology : JASN, Vol. 16, Issue 12, pp. 3661-8, (2005) (PubMed).
Kleijmeer, Ramm, Schuurhuis, Griffith, Rescigno, Ricciardi-Castagnoli, Rudensky, Ossendorp, Melief, Stoorvogel, Geuze: "Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells." dans: The Journal of cell biology, Vol. 155, Issue 1, pp. 53-63, (2001) (PubMed).
Clausen, Waldburger, Schwenk, Barras, Mach, Rajewsky, Förster, Reith: "Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5-deficient mice." dans: Immunity, Vol. 8, Issue 2, pp. 143-55, (1998) (PubMed).
De Souza Leao, Lang, Prina, Hellio, Antoine: "Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells." dans: Journal of cell science, Vol. 108 ( Pt 10), pp. 3219-31, (1995) (PubMed).
Viville, Neefjes, Lotteau, Dierich, Lemeur, Ploegh, Benoist, Mathis: "Mice lacking the MHC class II-associated invariant chain." dans: Cell, Vol. 72, Issue 4, pp. 635-48, (1993) (PubMed).
Bhattacharya, Dorf, Springer: "A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2488-95, (1982) (PubMed).
Antigène
MHC Class II (MHC2)
(Major Histocompatibility Complex Class II Antigen (MHC2))
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.