Nitrotyrosine anticorps

N° du produit ABIN1302502

Détail du produit anti-Nitrotyrosine anticorps

Antigène: Nitrotyrosine

Reactivité: Différentes espèces

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nitrotyrosine est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: The antibody EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues.

Réactivité croisée (Details): Broad

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: NO2-Tyr-CH2-Thyroglobulin

Clone: EM-30

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia.
Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia.
Western blotting: positive control: NO2-BSA, NO2-HSA.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Détails sur Nitrotyrosine

Antigène: Nitrotyrosine

Abstract: Nitrotyrosine Produits

Classe de substances: Chemical

Sujet: Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.,TyrNO₂, C₉H₁₀N₂O₅