GAPDHS anticorps (FITC)

N° du produit ABIN1302845

L’anticorps anti-GAPDHS Monoclonal Souris est utilisé pour la détection de GAPDHS dans des échantillons de Humain et Porc. Il a été validé pour FACS.

Aperçu rapide pour GAPDHS anticorps (FITC) (ABIN1302845)

Antigène: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Reactivité: Humain, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GAPDHS est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Clone: Hs-8

Détail du produit anti-GAPDHS anticorps (FITC)

Fonction: Anti-GAPDHS FITC

Réactivité croisée (Details): Human, Porcine

Attributs du produit: The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Isotype: IgM

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 50 tests. Intraacrosomal staining.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze,Protect from prolonged exposure to light

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Détails sur GAPDHS

Antigène: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Autre désignation: GAPDHS

Sujet: Glyceraldehyde-3-phosphate dehydrogenase, spermato,GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,SGAPDH, HSD-35

ID gène: 26330

UniProt: O14556

Pathways: Regulation of Carbohydrate Metabolic Process