CD8B
Reactivité: Humain
WB, IP
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature
Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Volume d'échantillon
1 mL
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
0.5 mg in 1.0 mL of PBS/Sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Protect conjugated products from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Stock
4 °C
Stockage commentaire
Store at 2-8°C
Lacharme-Lora, Chaloner, Gilroy, Humphrey, Gibbs, Jopson, Wright, Reid, Ketley, Humphrey, Williams, Rushton, Wigley: "B lymphocytes play a limited role in clearance of Campylobacter jejuni from the chicken intestinal tract." dans: Scientific reports, Vol. 7, pp. 45090, (2018) (PubMed).
Dalgaard, Norup, Pedersen, Handberg, Juorgensen, Juul-Madsen: "Flow cytometric assessment of chicken T cell-mediated immune responses after Newcastle disease virus vaccination and challenge." dans: Vaccine, (2010) (PubMed).
In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two chains, and (ii) a heterodimer of an chain and a chain.1 While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8- and CD8-chains, a relatively large proportion of the CD8+ TCR- cells in the spleens of embryos and young chicks express only the -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR- CD8 cells in the chicken that is not found in rodents.3 Chicken CD8 is expressed on approximately 80 % of thymocytes, 45 % of blood mononuclear cells and 50 % of spleen cells, but <1 % of cells in the bursa and bone marrow.