Chicken/Pigeon/Caiman MHC Class II β-chain, Mr 30-42 kDa
Attributs du produit
Mouse Anti-Chicken MHC Class II-PE
Purification
The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Applications: FC - Quality tested , IHC-FS - Reported in literature , EM - Reported in literature , IP - Reported in literature , Purification - Reported in literature
Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Volume d'échantillon
1 mL
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do not freeze! Protect conjugated products from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Stock
4 °C
Stockage commentaire
Store at 2-8°C
Weiss, Christensen, Zeuthen, Vogensen, Jakobsen, Frøkiær: "Lactobacilli and bifidobacteria induce differential interferon-β profiles in dendritic cells." dans: Cytokine, Vol. 56, Issue 2, pp. 520-30, (2011) (PubMed).
Antigène
MHC Class II (MHC2)
(Major Histocompatibility Complex Class II Antigen (MHC2))
The chicken major histocompatibility complex (MHC), or B complex, consists of several clusters of highly polymorphic genes. Like their mammalian counterparts, the avian MHC exerts genetic influence over a variety of important biological functions such as immune response, disease resistance, growth and development, aging, and reproduction. Chicken MHC Class II genes, also known as the B-L subregion, of the chicken MHC encode cell surface glycoproteins that are homologous to mammalian Class II antigens. B-L antigens structurally are similar to mammalian Class II molecules in that they are noncovalently bound dimmers of one 32-34 kDa heavy chain and one 27-30 kDa light chain. MHC Class II is primarily expressed on B cells and antigen presenting cells (APCs).