Bu-1 anticorps
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- Antigène Tous les produits Bu-1
- Bu-1
- Reactivité
- Poulet
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Bu-1 est non-conjugé
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Application
- Flow Cytometry (FACS)
- Specificité
- Chicken Bu-1, Mr 70 kDa
- Attributs du produit
- Mouse Anti-Chicken Bu-1-UNLB
- Purification
- Purified
- Immunogène
- Chicken peripheral blood lymphocytes and splenocytes
- Clone
- AV20
- Isotype
- IgG1
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- Indications d'application
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- Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature
- Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells AF647 conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
- Volume d'échantillon
- 1 mL
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
- Agent conservateur
- Without preservative
- Conseil sur la manipulation
- Each reagent is stable for the period shown on the bottle label if stored as directed.
- Stock
- 4 °C
- Stockage commentaire
- Store at 2-8°C
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Chickens with humanized immunoglobulin genes generate antibodies with high affinity and broad epitope coverage to conserved targets." dans: mAbs, Vol. 10, Issue 1, pp. 71-80, (2019) (PubMed).
: "The Postembryonic Development of the Immunological Barrier in the Chicken Spleens." dans: Journal of immunology research, Vol. 2019, pp. 6279360, (2019) (PubMed).
: "
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Chickens with humanized immunoglobulin genes generate antibodies with high affinity and broad epitope coverage to conserved targets." dans: mAbs, Vol. 10, Issue 1, pp. 71-80, (2019) (PubMed).
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- Antigène
- Bu-1
- Abstract
- Bu-1 Produits
- Synonymes
- anticorps B6.2, anticorps Bu-1, anticorps Bu-1b, anticorps B6.1, anticorps LOC396098
- Sujet
- Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated, disulfide-linked homodimer. MAb AV20 recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90 % of bursal cells, 2-8 % of thymocytes, 15-27 % of spleen cells, and 2-18 % of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.
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