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Bu-1 anticorps

Reactivité: Poulet FACS Hôte: Souris Monoclonal AV20 unconjugated
N° du produit ABIN135594
  • Antigène Tous les produits Bu-1
    Bu-1
    Reactivité
    Poulet
    Hôte
    • 9
    Souris
    Clonalité
    • 9
    Monoclonal
    Conjugué
    • 3
    • 2
    • 2
    • 2
    Cet anticorp Bu-1 est non-conjugé
    Application
    • 9
    • 6
    • 6
    • 5
    Flow Cytometry (FACS)
    Specificité
    Chicken Bu-1, Mr 70 kDa
    Attributs du produit
    Mouse Anti-Chicken Bu-1-UNLB
    Purification
    Purified
    Immunogène
    Chicken peripheral blood lymphocytes and splenocytes
    Clone
    AV20
    Isotype
    IgG1
  • Indications d'application
    • Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature
    • Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells AF647 conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
    Volume d'échantillon
    1 mL
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added
    Agent conservateur
    Without preservative
    Conseil sur la manipulation
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C
  • Ching, Collarini, Abdiche, Bedinger, Pedersen, Izquierdo, Harriman, Zhu, Etches, van de Lavoir, Harriman, Leighton: "Chickens with humanized immunoglobulin genes generate antibodies with high affinity and broad epitope coverage to conserved targets." dans: mAbs, Vol. 10, Issue 1, pp. 71-80, (2019) (PubMed).

    Zhang, Sun, Wang, Chen, Huang, Chen, Chen: "The Postembryonic Development of the Immunological Barrier in the Chicken Spleens." dans: Journal of immunology research, Vol. 2019, pp. 6279360, (2019) (PubMed).

  • Antigène
    Bu-1
    Abstract
    Bu-1 Produits
    Synonymes
    anticorps B6.2, anticorps Bu-1, anticorps Bu-1b, anticorps B6.1, anticorps LOC396098
    Sujet
    Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated, disulfide-linked homodimer. MAb AV20 recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90 % of bursal cells, 2-8 % of thymocytes, 15-27 % of spleen cells, and 2-18 % of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.
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