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CD8 alpha anticorps (AA 51-150)

Name: CD8 alpha anticorps (AA 51-150)
SKU: ABIN1387948
Price: 384.62 EUR
Availability: InStock

CD8A Reactivité: Humain, Rat, Poulet FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) Hôte: Lapin Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded rat spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD8 alpha Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (CD8 alpha anticorps (AA 51-150))

IHC (p)

Formalin-fixed and paraffin embedded rat colon labeled with Anti-CD8 alpha Polyclonal Antibody, Unconjugated (ABIN1387948) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. (CD8 alpha anticorps (AA 51-150))

IF (p)

3 images

(2 références)

N° du produit ABIN1387948

Fiche de données

Antigène Voir toutes CD8 alpha (CD8A) Anticorps

CD8 alpha (CD8A) (CD8a Molecule (CD8A))
Épitope
15

15

10

9

9

9

8

7

6

6

5

5

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 51-150
Reactivité
205

117

46

44

15

7

3

2

2

1

1

1

1

1

1

1

1
Humain, Rat, Poulet
Hôte
174

126

104
Lapin
Anticorps secondaires appropriés
Clonalité
298

102

1
Polyclonal
Conjugué
118

46

36

30

30

11

9

8

8

8

8

7

6

6

5

5

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp CD8 alpha est non-conjugé
Application
310

130

93

67

65

46

21

18

15

14

13

11

10

8

7

7

6

6

5

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Réactivité croisée

Poulet, Humain, Rat

Purification

Purified by Protein A.

Immunogène

KLH conjugated synthetic peptide derived from rat CD8 alpha

Isotype

IgG

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anti-CD8a Molecule (CD8A) antibody (FITC)
CD8A Reactivité: Souris FACS Hôte: Rat Monoclonal 53-6-7 FITC

anti-CD8a Molecule (CD8A) antibody (PE)
CD8A Reactivité: Souris FACS Hôte: Rat Monoclonal 53-6-7 PE

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CD8A Reactivité: Humain IHC, WB, IP, ELISA Hôte: Lapin Monoclonal 2F2 unconjugated Recombinant Antibody

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CD8A Reactivité: Humain FACS, IHC, WB, ELISA, ICC, Neut Hôte: Souris Monoclonal 5H5D7 unconjugated

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CD8A Reactivité: Humain IHC, StM, ISt Hôte: Souris Monoclonal C8-468 unconjugated

anti-CD8a Molecule (CD8A) (AA 28-196) antibody
CD8A Reactivité: Souris, Rat FACS, IHC, WB, ELISA, ICC Hôte: Lapin Polyclonal unconjugated

anti-CD8a Molecule (CD8A) (AA 150-180) antibody
CD8A Reactivité: Humain FACS, IHC, WB, ELISA Hôte: Lapin Polyclonal unconjugated

Indications d'application

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption

12 months
Savvatis, Müller, Fröhlich, Pappritz, Zietsch, Hamdani, Grote, Schieffer, Klingel, Van Linthout, Linke, Schultheiss, Tschöpe: "Interleukin-6 receptor inhibition modulates the immune reaction and restores titin phosphorylation in experimental myocarditis." dans: Basic research in cardiology, Vol. 109, Issue 6, pp. 449, (2014) (PubMed).

Cheng, Guo, Qiao, Liu, Nie, Li, Wang, Jiang: "T cell immunohistochemistry refines lung transplant acute rejection diagnosis and grading." dans: Diagnostic pathology, Vol. 8, pp. 168, (2013) (PubMed).
Antigène

CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Autre désignation

CD8 alpha (CD8A Produits)

Synonymes

anticorps cd8, anticorps LOC100136450, anticorps BB154331, anticorps Ly-2, anticorps Ly-35, anticorps Ly-B, anticorps Lyt-2, anticorps zgc:136643, anticorps CD8, anticorps Leu2, anticorps MAL, anticorps p32, anticorps RHACD8-4, anticorps CD8a molecule, anticorps uncharacterized LOC100125537, anticorps CD8 alpha, anticorps CD8 antigen, alpha chain, anticorps T-cell surface glycoprotein CD8 alpha chain, anticorps Cd8a, anticorps LOC100125537, anticorps LOC100136450, anticorps CD8A, anticorps cd8a, anticorps LOC100126556

Sujet

Synonyms: T-cell surface glycoprotein CD8 alpha chain, CD8 antigen 32 kDa chain, OX-8 membrane antigen, CD8a
Background: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

ID gène

24930

UniProt

P07725

Pathways

TCR Signaling