SETDB1 anticorps (AA 201-300) (AbBy Fluor® 350)
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- Antigène Voir toutes SETDB1 Anticorps
- SETDB1 (SET Domain, Bifurcated 1 (SETDB1))
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Épitope
- AA 201-300
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Reactivité
- Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp SETDB1 est conjugé à/à la AbBy Fluor® 350
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Application
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- Réactivité croisée
- Rat
- Homologie
- Human,Mouse,Dog,Cow,Sheep,Pig,Horse,Rabbit
- Purification
- Purified by Protein A.
- Immunogène
- KLH conjugated synthetic peptide derived from human SETDB1
- Isotype
- IgG
- Top Product
- Discover our top product SETDB1 Anticorps primaire
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- Indications d'application
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IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Agent conservateur
- ProClin
- Précaution d'utilisation
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- SETDB1 (SET Domain, Bifurcated 1 (SETDB1))
- Autre désignation
- KMT1E/SETDB1 (SETDB1 Produits)
- Synonymes
- anticorps ESET, anticorps H3-K9-HMTase4, anticorps KG1T, anticorps KMT1E, anticorps TDRD21, anticorps AU022152, anticorps mKIAA0067, anticorps SETDB1, anticorps NV10069, anticorps fc07d03, anticorps si:ch211-81a5.6, anticorps wu:fc07d03, anticorps im:7143091, anticorps wu:fa04a12, anticorps wu:fj80h02, anticorps zgc:152899, anticorps SET domain bifurcated 1, anticorps SET domain, bifurcated 1, anticorps myeloid/lymphoid or mixed-lineage leukemia; translocated to, 11, anticorps histone-lysine N-methyltransferase SETDB1, anticorps SET domain, bifurcated 1a, anticorps SET domain bifurcated 1 L homeolog, anticorps SET domain, bifurcated 1b, anticorps SETDB1, anticorps Setdb1, anticorps MLLT11, anticorps LOC100120014, anticorps LOC100461671, anticorps setdb1a, anticorps setdb1.L, anticorps setdb1b
- Sujet
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Synonyms: ESET, KG1T, KMT1E, TDRD21, H3-K9-HMTase4, Histone-lysine N-methyltransferase SETDB1, ERG-associated protein with SET domain, Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, Lysine N-methyltransferase 1E, SET domain bifurcated 1, SETDB1, KIAA0067
Background: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610).
- ID gène
- 9869
- UniProt
- Q15047
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