PDZRN3 anticorps (AbBy Fluor® 350)

N° du produit ABIN1401363

Détail du produit anti-PDZRN3 anticorps (AbBy Fluor® 350)

Antigène: PDZRN3 (PDZ Domain Containing Ring Finger 3 (PDZRN3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PDZRN3 est conjugé à/à la AbBy Fluor® 350

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Réactivité croisée: Humain, Souris, Rat

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human SEMCAP3/LNX3

Isotype: IgG

Information d'application

Indications d'application: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur PDZRN3

Antigène: PDZRN3 (PDZ Domain Containing Ring Finger 3 (PDZRN3))

Autre désignation: SEMCAP3 (PDZRN3 Produits)

Synonymes: anticorps MGC80196, anticorps pdzrn3, anticorps LNX3, anticorps SEMACAP3, anticorps 1110020C07Rik, anticorps AI429718, anticorps AL023082, anticorps Semcap3, anticorps PDZ domain containing ring finger 3, anticorps PDZ domain containing ring finger 3 L homeolog, anticorps PDZ domain containing RING finger 3b, anticorps E3 ubiquitin-protein ligase PDZRN3, anticorps PDZ domain containing RING finger 3, anticorps PDZRN3, anticorps pdzrn3.L, anticorps pdzrn3b, anticorps LOC100228989, anticorps pdzrn3, anticorps Pdzrn3

Sujet:

Synonyms: Ligand of Numb protein X 3, likely ortholog of mouse semaF cytoplasmic domain associated protein 3, LNX3, PDZ domain containing ring finger 3, Protein SEMACAP3, SEMACAP3, Semaphorin cytoplasmic domain associated protein, PZRN3_HUMAN.

Background: PDZRN3 contains a RING-finger motif in its N-terminal region, two PDZ domains in its central region and a consensus-binding motif for PDZ domains at its C-terminus. It was identified in silico as a homolog of the protein known as LNX1 or SEMCAP1, which possesses ubiquitin ligase activity and binds the membrane protein Semaphorin 4C. However, PDZRN3 itself has not previously been characterized. We have now evaluated the properties and functions of PDZRN3. The PDZRN3 gene was shown to be expressed in various human tissues including the heart, skeletal muscle and liver and its expression in mouse skeletal muscle was developmentally regulated. Both the differentiation of C2C12 mouse skeletal myoblasts into myotubes and injury-induced muscle regeneration in vivo were found to be accompanied by up-regulation of PDZRN3. The differentiation-associated increase in the expression of PDZRN3 in C2C12 cells follows that of myogenin and precedes that of myosin heavy chain. Depletion of PDZRN3 by RNA interference inhibited the formation of myotubes as well as the associated up-regulation of myosin heavy chain in C2C12 cells. Our data suggest that PDZRN3 plays an essential role in the differentiation of myoblasts into myotubes by acting either downstream or independently of myogenin.

ID gène: 23024

Pathways: Skeletal Muscle Fiber Development