Prostate Specific Antigen anticorps

N° du produit ABIN1543584

Détail du produit anti-Prostate Specific Antigen anticorps

Antigène: Prostate Specific Antigen (PSA)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Prostate Specific Antigen est non-conjugé

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Dot Blot (DB)

Purification: Protein A affinity column

Immunogène: Native PSA Antigen

Clone: A5D5-1

Isotype: IgG1

Information d'application

Commentaires:

Other Applications have not been tested.

Restrictions: For Research Use only

Independent Validation (FACS)

Validation #029617 (Flow Cytometry)

by: Flow Cytometry & Cell Separation Facility, Purdue University

No.: #029617

Date: 22.02.2014

Numéro du lot: FAPS-A5D5-0003

Application validée: Flow Cytometry

Contrôle positif: LNCaP cells

Contrôle négative: - DU145 cells - Jurkat cells

Conclusion: Strong signal above isotype signal was observed in the positive control sample, while staining of similar level to isotype control was observed in both negative control samples.

Validation Images

Figure 1: LNCaP cells stained with anti-PSA (blue) or with isotype control (red).

Figure 2: DU145 cells stained with anti-PSA (blue) or with isotype control (red).

Figure 3: Jurkat cells stained with anti-PSA (blue) or with isotype control (red).

Protocole

Anticorps primaire:

• Antibody: Prostate Specific Antigen (PSA)
• Catalog number: ABIN1543584
• Lot number: FAPS-A5D5-0003

Anticorps secondaire:

• Antibody: F(ab’)2 goat anti-mouse IgG-Alexa 647

Full Protocol:

• Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with 0.05% trypsin / 0.025% EDTA.
• Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
• An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37°C.
• Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
• Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA and 0.1% Tween 20
• Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA.
• 1 µg of anti-PSA or mouse IgG1 isotype control were added to the cells and incubated for 30 min at room temperature
• Cells were washed twice with PBS/BSA/Tween 20
• Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA
• Secondary antibody was added at a final dilution of 1:500 and incubated for 30 min at room temperature
• Cells were washed twice with PBS/BSA/Tween 20
• Cells were resuspended in 0.3 mL PBS/BSA
• Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).

Notes: None

Stockage

Format: Liquid

Concentration: 1.8 mg/mL

Buffer: PBS, pH 7.4, 0.09% sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détails sur Prostate Specific Antigen

Antigène: Prostate Specific Antigen (PSA)

Autre désignation: PSA (PSA Produits)

Synonymes: anticorps APS, anticorps KLK2A1, anticorps PSA, anticorps hK3, anticorps KLK3, anticorps Klk1c10l2, anticorps RSGK-50, anticorps RSKG-50, anticorps rGK-3, anticorps Kal-3, anticorps Kal3, anticorps Klk3, anticorps AAP-S, anticorps MP100, anticorps Psa, anticorps R74825, anticorps goku, anticorps kallikrein related peptidase 3, anticorps kallikrein 3, anticorps kallikrein 1-related peptidase C3, anticorps kallikrein B, plasma 1, anticorps aminopeptidase puromycin sensitive, anticorps KLK3, anticorps Klk1c3, anticorps Klkb1, anticorps Npepps

Pathways: Système du Complément