Flow Cytometry (FACS), Western Blotting (WB), ELISA, Functional Studies (Func)
Attributs du produit
The antibody recognizes an epitope within the a2 domain of the human high affinity IgE receptor (FceRIa). It inhibits binding of IgE to this receptor and is suitable to detect receptors expressed on primary cells and cell lines (e.g mast cells and basophils). It can also be used as weak to moderate cross-linking agent for IgE-independent triggering of effector cells and to detect FceRIa in ELISA and W-blots.
Purification
Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on anti-mouse IgG1 agarose
Can be used for functional mast cell / basophil assays
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
PBS, pH=7.2, 0.1 % NaN3
Agent conservateur
Sodium azide
Précaution d'utilisation
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Stock
4 °C/-20 °C
Stockage commentaire
Store undiluted at 2-8°C. For long term storage, aliquot and store at -20°C or -80°C
Antigène
Fc epsilon RI/FCER1A (FCER1A)
(Fc Fragment of IgE Receptor Ia (FCER1A))