Protein C Tag anticorps

N° du produit ABIN1573910

Détail du produit anti-Protein C Tag anticorps

Antigène: Protein C Tag

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Protein C Tag est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: This Antibody recognizes C-terminal, N-terminal, and internal Protein C tagged fusion proteins.

Purification: Immunoaffinity chromatography

Immunogène: Protein C epitope tag peptide CEDQVDPRLIDGK conjugated - KLH

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (Rabbit) is recommended. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm X 8 cm membrane.Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 %Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Références pour anticorps anti-Protein C Tag (ABIN1573910)

Wong, Bienzle, Hayes, Taylor, Wood: "Purification of protein C from canine plasma." dans: BMC veterinary research, Vol. 10, pp. 251, (2015) (PubMed).

Wolf, Reimer, Schuck, Rüder, Gerlach, Müller, Otto, Dörken, Rehm: "Role of EBAG9 protein in coat protein complex I-dependent glycoprotein maturation and secretion processes in tumor cells." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 10, pp. 4000-19, (2010) (PubMed).

Détails sur Protein C Tag

Antigène: Protein C Tag

Abstract: Protein C Tag Produits

Classe de substances: Tag

Sujet: Rabbit Anti-Protein C (HPC4)-tag is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH7.4, containing 0.02% Sodium azide.