Protein A anticorps (HRP)

N° du produit ABIN1573943

Détail du produit anti-Protein A anticorps (HRP)

Antigène: Protein A

Reactivité: Staphylococcus aureus

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp Protein A est conjugé à/à la HRP

Application: ELISA, Western Blotting (WB)

Specificité: Chicken Anti-Protein A [HRP] Polyclonal Antibody is specific to protein A.

Immunogène: Purified recombinant protein A

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.05-1.0 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Références pour anticorps anti-Protein A (HRP) (ABIN1573943)

OHalloran, Wynne, Geoghegan: "Protein A is released into the Staphylococcus aureus culture supernatant with an unprocessed sorting signal." dans: Infection and immunity, Vol. 83, Issue 4, pp. 1598-609, (2015) (PubMed).

Nguyen, Rocha, Chintalacharuvu, Beenhouwer: "Detection and quantification of Panton-Valentine leukocidin in Staphylococcus aureus cultures by ELISA and Western blotting: diethylpyrocarbonate inhibits binding of protein A to IgG." dans: Journal of immunological methods, Vol. 356, Issue 1-2, pp. 1-5, (2010) (PubMed).

Kathirvel, Morgan, French, Morgan: "Overexpression of liver-specific cytochrome P4502E1 impairs hepatic insulin signaling in a transgenic mouse model of nonalcoholic fatty liver disease." dans: European journal of gastroenterology & hepatology, Vol. 21, Issue 9, pp. 973-83, (2009) (PubMed).

Détails sur Protein A

Antigène: Protein A

Autre désignation: ProteinA (Protein A Produits)

Synonymes: anticorps alpha protein, anticorps repB

Sujet: Chicken Anti-Protein A [HRP] Polyclonal Antibody is developed in chicken host animals using purified recombinant protein A. Chicken anti-Protein A [HRP] is highly purified from chicken yolks by immunoaffinity chromatography and then conjugated to horseradish peroxidase. Chicken anti-Protein A is suitable for ELISA and immunoblotting.