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Geminin anticorps (AA 50-100)

GMNN Reactivité: Humain WB, ELISA, FACS Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1574066
  • Antigène Voir toutes Geminin (GMNN) Anticorps
    Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))
    Épitope
    • 9
    • 7
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 50-100
    Reactivité
    • 54
    • 11
    • 10
    Humain
    Hôte
    • 46
    • 10
    Lapin
    Clonalité
    • 44
    • 12
    Polyclonal
    Conjugué
    • 31
    • 5
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Geminin est non-conjugé
    Application
    • 31
    • 25
    • 24
    • 12
    • 8
    • 7
    • 7
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Flow Cytometry (FACS)
    Specificité
    Geminin Antibody, pAb, Rabbit detects endogenous levels of human geminin protein.
    Purification
    Immunoaffinity chromatography
    Immunogène
    KLH-coupled synthetic peptide within AA 50-100 of human geminin protein .
    Isotype
    IgG
    Top Product
    Discover our top product GMNN Anticorps primaire
  • Indications d'application
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    ELISA: 0.05-0.2 µg/mL
    Western blot: 0.5-1 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther applications: user-optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4, containing 0.02 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Wang, Kirschner: "Emi1 preferentially inhibits ubiquitin chain elongation by the anaphase-promoting complex." dans: Nature cell biology, Vol. 15, Issue 7, pp. 797-806, (2013) (PubMed).

  • Antigène
    Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))
    Autre désignation
    Geminin (GMNN Produits)
    Synonymes
    anticorps CG3183, anticorps Dm Geminin, anticorps Dm geminin, anticorps Dmel\\CG3183, anticorps Gem, anticorps Geminin, anticorps anon-WO0257455.1, anticorps gem, anticorps l(2)k14019, anticorps v(2)k09107, anticorps sb:cb254, anticorps zgc:66217, anticorps GB16871, anticorps GMNN, anticorps geminin, anticorps dyak_GLEANR_833, anticorps DyakGE24654, anticorps GE24654, anticorps LOC100049517, anticorps AW546347, anticorps geminin, DNA replication inhibitor, anticorps CG3183 gene product from transcript CG3183-RA, anticorps geminin, anticorps GE24654 gene product from transcript GE24654-RA, anticorps GMNN, anticorps geminin, anticorps gmnn, anticorps LOC410093, anticorps Dyak\geminin, anticorps Gmnn
    Sujet
    Geminin inhibits DNA replication by preventing the incorporation of the MCM complex into the prereplication complex (pre-RC). It is absent during the G1 phase, accumulates during the S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition. Geminin contains 212 amino acids and has a destruction box sequence of RRTLKVIQP. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.Geminin Antibody, pAb, Rabbit is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 50-100 of human geminin protein (Swiss Prot: O75496).
    Pathways
    EGFR Signaling Pathway, DNA Replication
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