GFAP anticorps (AA 250-300)

N° du produit ABIN1574085

Détail du produit anti-GFAP anticorps

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Épitope: AA 250-300

Reactivité: Humain, Rat, Orang-Utan

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GFAP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificité: Rabbit Anti-GFAP Polyclonal Antibody detects endogenous levels of human and rat GFAP. It is predicted to react with sumatran orangutan GFAP protein according to sequence homology.

Réactivité croisée (Details): Rabbit Anti-GFAP Polyclonal Antibody detects endogenous levels of human and rat GFAP. It is predicted to react with sumatran orangutan GFAP protein according to sequence homology.

Purification: Immunoaffinity chromatography

Immunogène: KLH-coupled peptide from within AA 250-300 of human GFAP.

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLImmunohistochemistry: 5-10 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Références pour anticorps anti-GFAP (ABIN1574085)

Long, Watts, Li, Shen, Muir, Huang, Boggs, Suri, Duong: "The effects of perturbed cerebral blood flow and cerebrovascular reactivity on structural MRI and behavioral readouts in mild traumatic brain injury." dans: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, Vol. 35, Issue 11, pp. 1852-61, (2015) (PubMed).

Talley Watts, Shen, Deng, Chemello, Duong: "Manganese-Enhanced Magnetic Resonance Imaging of Traumatic Brain Injury." dans: Journal of neurotrauma, Vol. 32, Issue 13, pp. 1001-10, (2015) (PubMed).

Détails sur GFAP

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Autre désignation: GFAP (GFAP Produits)

Synonymes: anticorps GFAP, anticorps AI836096, anticorps cb345, anticorps etID36982.3, anticorps gfapl, anticorps wu:fb34h11, anticorps wu:fk42c12, anticorps xx:af506734, anticorps zgc:110485, anticorps glial fibrillary acidic protein, anticorps GFAP, anticorps LOC100136168, anticorps gfap, anticorps Gfap

Sujet: GFAP (Glial fibrillary acidic protein) is a member of intermediate filament (IF) protein family which consists mainly of cytokeratin, GFAP, desmin, and vimentin. Like other IF proteins, GFAP is composed of an amino terminal head domain, a central rod domain, and a carboxyl terminal tail domain. The amino terminal region of GFAP shares considerable sequence divergence with other IF proteins, and probably participates in mediation of the tissue specific functions. GFAP intermediate filament serves as a useful marker of astroglia in the brain since it is specifically expressed in astrocytes and certain other astroglia. This protein may be required for communications between Bergmann glia and Purkinje cells during long-term depression induction and maintenance.Rabbit Anti-GFAP Polyclonal Antibody is developed in rabbit using a synthetic KLH-coupled peptide from within 250-300 of human GFAP (Swiss Prot: P14136).