ENO2/NSE anticorps (C-Term)

N° du produit ABIN1574104

Détail du produit anti-ENO2/NSE anticorps

Antigène: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Épitope: C-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ENO2/NSE est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Specificité: Rabbit Anti-NSE Polyclonal Antibody detects endogenous levels of human, mouse, and rat NSE protein. It may cross-reacts with alpha and beta enolase according to sequence homology.

Réactivité croisée (Details): Rabbit Anti-NSE Polyclonal Antibody detects endogenous levels of human, mouse, and rat NSE protein. It may cross-reacts with alpha and beta enolase according to sequence homology.

Purification: Immunoaffinity chromatography

Immunogène: KLH-coupled synthetic peptide from C-terminal of human NSE

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLImmunoprecipitation (IP): 2-10 µg/mg of lysateImmunohistochemistry: 5-10 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Détails sur ENO2/NSE

Antigène: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Autre désignation: NSE (ENO2 Produits)

Synonymes: anticorps ENO2, anticorps DKFZp459B1817, anticorps NSE, anticorps AI837106, anticorps D6Ertd375e, anticorps Eno-2, anticorps RNEN3, anticorps eno3, anticorps wu:fc09h05, anticorps zgc:92418, anticorps enolase 2, anticorps enolase 2 (gamma, neuronal), anticorps enolase 2, gamma neuronal, anticorps enolase 2, gamma, neuronal, anticorps ENO2, anticorps Eno2, anticorps eno2

Sujet: Enolase is glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase has three subunits (alpha, beta, and gamma) that can form homo and heterodimers. Homodimers of gamma enolase are neuronal-specific. NSE (neuron specific enolase) is expressed primarily in neurons. It is also found in elevated levels in plasma in certain neoplasias including pediatric neuroblastoma and small cell lung cancer. NSE is widely used as a diagnostic marker in a variety of clinical assays.Rabbit Anti-NSE Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human NSE (Swiss Prot: P09104).