INA anticorps

N° du produit ABIN1580440

Détail du produit anti-INA anticorps

Antigène: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Reactivité: Humain, Mammifères

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp INA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purification: conc. tissue culture supernatant

Clone: 2E3

Isotype: IgG1

Information d'application

Indications d'application: For immunofluorescence try dilutions of 1:500, for immunohistochemistry with ABC or other enzymatic amplification procedures try 1:5,000. For western blots try 1:10,000 dilutions. On Western blots the antibody is unusually strong and specific in bindingas shown above. The antibody is clean and specific on immunoblots. Can be used on formalin-fixed cells in tissue culture, cryostat sections, and Western blotting. The epitope recognized by the 2E3 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so that this antibody is ideal for studies of paraffin embedded formalin fixed histological sections.

Restrictions: For Research Use only

Stockage

Format: Liquid

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 4°C short term or -20°C long term.

Détails sur INA

Antigène: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Abstract: INA Produits

Synonymes: anticorps NEF5, anticorps NF-66, anticorps TXBP-1, anticorps Inexa, anticorps Intlaa, anticorps Nf66, anticorps AV028420, anticorps NF66, anticorps ina-A, anticorps xefiltin, anticorps ina-a, anticorps inexa, anticorps MGC82496, anticorps INA, anticorps si:ch211-67n3.6, anticorps nef5, anticorps xina, anticorps nf-66, anticorps txbp-1, anticorps MGC69277, anticorps gefiltin, anticorps zgc:110516, anticorps internexin neuronal intermediate filament protein alpha, anticorps internexin neuronal intermediate filament protein, alpha, anticorps internexin neuronal intermediate filament protein alpha S homeolog, anticorps low molecular weight neuronal intermediate filament S homeolog, anticorps internexin neuronal intermediate filament protein, alpha a, anticorps internexin neuronal intermediate filament protein, alpha b, anticorps INA, anticorps Ina, anticorps ina.S, anticorps nif.S, anticorps inaa, anticorps ina, anticorps inab

Sujet: A-internexin is a Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits. On SDS-PAGE gels it runs with an apparent molecular weight of 64 to 66 kDa, with some species variability, although the real molecular weight is about 55 kDa, as with the other neurofilament subunits the presence of highly negatively charged sequences results in reduction of SDS-PAGE mobility. a-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development procedes. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to a-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. In addition recent studies show a marked up-regulation of a-internexin during neuronal regeneration. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Trojanowski, Lee and coworkers used this antibody to show that a-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID, see two Cairns et al. papers listed below), a serious human neurodegenerative disorder. The antibody was also used to confirm the presence of circulating auto-antibodies to a-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (Rajasalu et al., 2004, listed below). The HGNC name for this protein is INA.