ITK anticorps (Internal Region)

N° du produit ABIN1589997

Détail du produit anti-ITK anticorps

Antigène: ITK (IL2-Inducible T-Cell Kinase (ITK))

Épitope: Internal Region

Reactivité: Humain, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ITK est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Fonction: ITK

Séquence: KERPEDRPAF SR

Réactivité croisée: Boeuf (Vache), Humain, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 70 kDa band observed in Rat Colon, Lung and Spleen lysates (calculated MW of 72.4 kDa according to Rat NP_001102295.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of Jurkat cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concen

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur ITK

Antigène: ITK (IL2-Inducible T-Cell Kinase (ITK))

Autre désignation: ITK (ITK Produits)

Synonymes: anticorps Emt, anticorps Tcsk, anticorps Tsk, anticorps EMT, anticorps LPFS1, anticorps LYK, anticorps PSCTK2, anticorps IL2 inducible T cell kinase, anticorps IL2 inducible T-cell kinase, anticorps IL2-inducible T-cell kinase, anticorps Itk, anticorps ITK

Sujet: ITK, IL2-inducible T-cell kinase, EMT, LYK, MGC126257, MGC126258, PSCTK2, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK, tyrosine-protein kinase LYK

ID gène: 3702

NCBI Accession: NP_005537

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway