PKC beta anticorps (Internal Region)

N° du produit ABIN1590084

Détail du produit anti-PKC beta anticorps

Antigène: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Épitope: Internal Region

Reactivité: Humain, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PKC beta est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Fonction: PRKCB

Séquence: DKEFTRQPVE LT

Specificité: This antibody is expected to recognize isoform 1 (NP_997700.1) only.

Réactivité croisée: Chien, Humain, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration, 1-2 μg/mL.

Western Blot: Approx 80 kDa band observed in lysates of Human Placenta and Rat Brain (calculated MW of 76.9 kDa according to NP_997700.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur PKC beta

Antigène: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Autre désignation: PRKCB (PRKCB Produits)

Synonymes: anticorps PKC-beta, anticorps PKCB, anticorps PRKCB1, anticorps PRKCB2, anticorps A130082F03Rik, anticorps PKC-Beta, anticorps Pkcb, anticorps Prkcb1, anticorps Prkcb2, anticorps PKC-B, anticorps Prkcb, anticorps prkcb1, anticorps zgc:63591, anticorps PKC, anticorps PRKCB_tv2, anticorps Pkc53E, anticorps prkcb1l, anticorps zgc:64063, anticorps protein kinase C beta, anticorps protein kinase C, beta, anticorps protein kinase C, beta b, anticorps protein kinase C, beta a, anticorps PRKCB, anticorps Prkcb, anticorps prkcbb, anticorps prkcba

Sujet: PRKCB, protein kinase C, beta, MGC41878, PKC-beta, PKCB, PRKCB1, PRKCB2, OTTHUMP00000162377, OTTHUMP00000162378, PKC-B, protein kinase C beta type, protein kinase C, beta 1 polypeptide

ID gène: 5579

NCBI Accession: NP_997700

Pathways: Signalisation WNT, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling