KIF11 anticorps

N° du produit ABIN1607602

Détail du produit anti-KIF11 anticorps

Antigène: KIF11 (Kinesin Family Member 11 (KIF11))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KIF11 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Réactivité croisée (Details): Cross reactivity with Eg5 from other sources have not been determined.

Pureté: Anti-Eg5 is directed against the human Eg5 protein. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest reactivity with human.

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Eg5 was prepared from whole rabbit serum produced by repeated immunizations with a truncated Eg5 construct expressed in E. coli corresponding to human Eg5 protein.
Immunogen Type: Recombinant Protein

Information d'application

Indications d'application: Eg5 antibody has been tested for use in ELISA and western blot. For western blots expect a band of approximately 72 kDa in size corresponding to truncated kinesin-1 protein. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:10.000
Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 46 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur KIF11

Antigène: KIF11 (Kinesin Family Member 11 (KIF11))

Autre désignation: Eg5 (KIF11 Produits)

Synonymes: anticorps EG5, anticorps HKSP, anticorps KNSL1, anticorps MCLMR, anticorps TRIP5, anticorps Eg5, anticorps Kif8, anticorps Kifl1, anticorps Knsl1, anticorps F2H17.19, anticorps F2H17_19, anticorps DDBDRAFT_0189194, anticorps DDBDRAFT_0201556, anticorps DDB_0189194, anticorps DDB_0201556, anticorps XLEg5K1, anticorps XlEg5, anticorps cos2, anticorps costal2, anticorps eg5, anticorps hksp, anticorps kif11, anticorps kif11-a, anticorps kif11-b, anticorps kif11b, anticorps knsl1, anticorps trip5, anticorps Cos2, anticorps Costal2, anticorps XLEg5K2, anticorps kif11a, anticorps kinesin family member 11, anticorps P-loop containing nucleoside triphosphate hydrolases superfamily protein, anticorps kinesin family member 11 S homeolog, anticorps kinesin family member 11 L homeolog, anticorps KIF11, anticorps Kif11, anticorps AT2G36200, anticorps kif11, anticorps kif11.S, anticorps kif11.L

Sujet: Eg5 (also known as kinesin-5 and KIF11) is a homo-tetramer which cross-links anti-parallel microtubules in the mitotic spindle to maintain spindle bipolarity. Eg5, a member of the Kinesin-5 subclass of kinesins, is a plus-end-directed tetrameric kinesin-family protein that influences the assembly and organization of the mitotic spindle, a self-assembled and dynamic microtubule-based structure that orchestrates chromosome segregation in dividing cells. Eg5 action is essential: when it is depleted from the cytoplasm of meiotically-mature Xenopus laevis eggs, abnormal monopolar spindles form, preventing successful division. Eg5 is expressed in all cells during mitosis and in post-mitotic neurons during development. In developing neurons pharmacological inhibition and siRNA knockdown of Eg5 results in longer axons, more branches, fewer bouts of axon retraction and the inability of growth cones to turn on contact with repulsive substrates. In migratory neurons, inhibition of Eg5 causes neurons to migrate in a random pattern and form shorter leading processes. In adult neurons, Eg5 has a similar effect on inhibiting the rate of short microtubule transport so pharmacological inhibition of adult Eg5 (i.e Monastrol) may be a potential therapeutic tool for the augmentation of adult axon regeneration. Synonyms: Kinesin-5, KIF11

ID gène: 3799

NCBI Accession: NP_004512

UniProt: P33176