HCP anticorps
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- Antigène Tous les produits HCP
- HCP (Host Cell Proteins (HCP))
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Reactivité
- E. coli
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp HCP est non-conjugé
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Application
- Western Blotting (WB), ELISA
- Fonction
- Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli.
- Specificité
- E.coli HCP Antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins. This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. The anti-sera reactivity is directed against E.coli Host Cell Proteins.
- Réactivité croisée (Details)
- Cross reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
- Attributs du produit
- Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate. Synonyms: Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, ECP antibody, Anti-ECP
- Purification
- This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. Reactivity is directed against E.coli Host Cell Proteins.
- Stérilité
- Sterile filtered
- Immunogène
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Anti-Host Cell Proteins Antibody was prepared combining antibodies that have been separatly developed by repeated immunizations with a mixture of high molecular weight (HMW) and a mixture of low molecular weight (LMW) proteins from E.Coli.
Immunogen Type: Native Protein
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- Indications d'application
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Western Blot Dilution: 1:500 - 1:1000
ELISA : 1:1000-1:2000 - Commentaires
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Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and is suitable for western blotting and ELISA.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 77.5 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store Host Cell Proteins Antibody at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
- Date de péremption
- 12 months
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- Antigène
- HCP (Host Cell Proteins (HCP))
- Autre désignation
- Host Cell Proteins (HCP Produits)
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