Glucoamylase anticorps (Biotin)

N° du produit ABIN1607644

Détail du produit anti-Glucoamylase anticorps (Biotin)

Antigène: Glucoamylase

Reactivité: Rhizopus

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Glucoamylase est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivity against Glucoamylase from other sources is unknown.

Pureté: Anti-Glucoamylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glucoamylase [Rhizopus].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Glucoamylase [Rhizopus]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Glucoamylase has been assayed against 1.0 µg of Glucoamylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15.000 to 1:75.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur Glucoamylase

Antigène: Glucoamylase

Abstract: Glucoamylase Produits

Synonymes: anticorps An03g06550, anticorps AO090003000321, anticorps AO090010000746, anticorps glucoamylase, anticorps SACE_7136, anticorps UREG_05278, anticorps RR_RS12725, anticorps ANI_1_820034, anticorps AOR_1_552154, anticorps AOR_1_1262024, anticorps PAAG_02688, anticorps MCYG_06367, anticorps VDBG_01600, anticorps VDBG_06382, anticorps MGYG_06815, anticorps TERG_07238

Sujet: Anti-Glucoamylase antibody recognizes the enzyme glucoamylase. The catalytic domain of glucoamylase hydrolyzes terminal 1,4-alpha-D-glucose from non-reducing ends and releases beta-glucose. Synonyms: 1 4 alpha D glucan glucohydrolase antibody, Amyloglucosidase antibody, Gluc 1 antibody, Gluc 2 antibody, Gluc 3 antibody, Glucan 1 4 alpha glucosidase antibody, Glucan 14 alpha glucosidase antibody

UniProt: P07683