Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Pyranose Oxidase anticorps (Biotin)

Reactivité: Microbial IP, WB, ELISA Hôte: Chèvre Polyclonal Biotin
N° du produit ABIN1607646
  • Antigène Tous les produits Pyranose Oxidase
    Pyranose Oxidase
    Reactivité
    Microbial
    Hôte
    • 3
    Chèvre
    Clonalité
    • 3
    Polyclonal
    Conjugué
    • 2
    • 1
    Cet anticorp Pyranose Oxidase est conjugé à/à la Biotin
    Application
    Immunoprecipitation (IP), Western Blotting (WB), ELISA
    Réactivité croisée (Details)
    Cross reactivity against Pyranose Oxidase from other sources is unknown.
    Pureté
    Anti-Pyranose oxidase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyranose Oxidase [E.coli].
    niveau d'endotoxine
    Low Endotoxin : No
    Immunogène
    Pyranose Oxidase [E.coli]
    Immunogen Type: Native Protein
    Isotype
    IgG
  • Indications d'application
    Anti-Pyranose oxidase antibody has been assayed against 1.0 µg of Pyranose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:70.000 to 1:350.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:20.000
    IF Immunoprecipitation Dilution: 1:100
    Western Blot Dilution: 1:500 - 1:5.000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    10 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Conseil sur la manipulation
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Date de péremption
    Expiration date is one (1) year from date of opening.
  • Antigène
    Pyranose Oxidase
    Abstract
    Pyranose Oxidase Produits
    Synonymes
    anticorps Pyranose oxidase, anticorps pyranose oxidase, anticorps Achl_3529, anticorps Kvar_1112, anticorps AMED_5392
    Sujet
    Anti-Pyranose oxidase recognizes the oxidoreductase pyranose oxidase. In general, pyranose catalyzes the oxidation of aldopyranoses at the carbon 2 position to form 2-ketoaldoses . Notably, pyranose oxidase catalyzes the conversion of D-glucose and oxygen to 2-dehydro-D-glucose and hydrogen peroxide using flavin adenine dinucleotide (FAD) as a cofactor. Pyranose oxidase also plays a role in the pentose phosphate pathway. Synonyms: P2Ox Pyranose oxidase PROD POD POx EC=1.1.3.10 Pyranose:oxygen 2-oxidoreductase Glucose 2-oxidase FAD-oxidoreductase
    UniProt
    Q5G234, P79076
Vous êtes ici:
Support technique