Cet anticorp Pyranose Oxidase est conjugé à/à la Biotin
Application
Immunoprecipitation (IP), Western Blotting (WB), ELISA
Réactivité croisée (Details)
Cross reactivity against Pyranose Oxidase from other sources is unknown.
Pureté
Anti-Pyranose oxidase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyranose Oxidase [E.coli].
niveau d'endotoxine
Low Endotoxin : No
Immunogène
Pyranose Oxidase [E.coli] Immunogen Type: Native Protein
Reactivité: E. coli
IP, WB, ELISA
Hôte: Chèvre
Polyclonal
unconjugated
Indications d'application
Anti-Pyranose oxidase antibody has been assayed against 1.0 µg of Pyranose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:70.000 to 1:350.000 of the reconstitution concentration is suggested for this product. ELISA Dilution: 1:5.000 - 1:20.000 IF Immunoprecipitation Dilution: 1:100 Western Blot Dilution: 1:500 - 1:5.000
Restrictions
For Research Use only
Format
Liquid
Concentration
10 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Conseil sur la manipulation
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Date de péremption
Expiration date is one (1) year from date of opening.
Anti-Pyranose oxidase recognizes the oxidoreductase pyranose oxidase. In general, pyranose catalyzes the oxidation of aldopyranoses at the carbon 2 position to form 2-ketoaldoses . Notably, pyranose oxidase catalyzes the conversion of D-glucose and oxygen to 2-dehydro-D-glucose and hydrogen peroxide using flavin adenine dinucleotide (FAD) as a cofactor. Pyranose oxidase also plays a role in the pentose phosphate pathway. Synonyms: P2Ox Pyranose oxidase PROD POD POx EC=1.1.3.10 Pyranose:oxygen 2-oxidoreductase Glucose 2-oxidase FAD-oxidoreductase