PFKM anticorps (Biotin)

N° du produit ABIN1607670

Détail du produit anti-PFKM anticorps (Biotin)

Antigène: PFKM (phosphofructokinase, Muscle (PFKM))

Reactivité: Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PFKM est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): Cross reactivity against Fructose-6-Phosphate Kinase from other sources may occur but have not been specifically determined.

Pureté: Anti-Fructose-6-Phosphate Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit Muscle].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Fructose-6-Phosphate Kinase [Rabbit Muscle]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Fructose-6-Phosphate Kinase antibody has been tested for use in ELISA, immunofluorescence microscopy and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract.
ELISA Dilution: 1:4.000 - 1:20.000
Western Blot Dilution: 1:500 - 1:2.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur PFKM

Antigène: PFKM (phosphofructokinase, Muscle (PFKM))

Autre désignation: Fructose-6-Phosphate Kinase (PFKM Produits)

Synonymes: anticorps GSD7, anticorps PFK-1, anticorps PFK1, anticorps PFKA, anticorps PFKX, anticorps AI131669, anticorps PFK-A, anticorps PFK-M, anticorps Pfk-4, anticorps Pfk4, anticorps Pfka, anticorps Pfkx, anticorps Pfk-M, anticorps M-PFK, anticorps PFKM, anticorps pfkm, anticorps zgc:92344, anticorps pfk, anticorps im:7137110, anticorps wu:fc58g11, anticorps phosphofructokinase, muscle, anticorps phosphofructokinase, platelet, anticorps phosphofructokinase, muscle a, anticorps phosphofructokinase, muscle S homeolog, anticorps phosphofructokinase, muscle b, anticorps PFKM, anticorps Pfkm, anticorps PFKP, anticorps pfkma, anticorps pfkm.S, anticorps pfkmb

Sujet: Fructose-6-Phosphate Kinase -2 (F6PK) also known as Phosphofructokinase (PFK) catalyzes the conversion of ATP + D-fructose 6-phosphate to ADP + D-fructose 1,6-bisphosphate and therefore is a key enzyme in the control of glycolysis and carbohydrate degradation.  This is a unidirectional and rate-limiting step in glycolysis.  Allosteric kinetics control activation by ADP, AMP, or fructose bisphosphate and inhibition by ATP or citrate.  The enzyme exists as a homotetramer. Synonyms: 6 Phosphofructokinase Muscle Type antibody, EC 2.7.1.11 antibody, GSD7 antibody, MGC8699 antibody, PFKA antibody, PFKL antibody, PFKM antibody, PFKP antibody, PFKX antibody, Phosphofructo 1 Kinase Isozyme A antibody, Phosphofructokinase 1 antibody

ID gène: 100345647, 125128

UniProt: P00511

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, L'effet Warburg