Pepsin anticorps (Biotin)

N° du produit ABIN1607675

Détail du produit anti-Pepsin anticorps (Biotin)

Antigène: Pepsin

Reactivité: Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Pepsin est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Réactivité croisée (Details): Cross reactivity against Pepsin from other sources may occur but have not been specifically determined.

Pureté: Anti-Pepsin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Pepsin [Porcine Stomach]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Pepsin antibody is suitable for ELISA, Immunohisochemistry, and Western Blot. Expect band at 41.2 kDa in appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:3.000 - 1:15.000
Western Blot Dilution: 1:500 - 1:2.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur Pepsin

Antigène: Pepsin

Abstract: Pepsin Produits

Sujet: Pepsin antibody detects Pepsin protein. Pepsin is an enzyme whose pepsinogen is released by the chief cells in the stomach and degrades food proteins into peptides. Pepsin belongs to the peptidase A1 family and is one of three principal proteolytic enzymes in the digestive system. Pepsin is most efficient in cleaving peptide bonds between hydrophobic and preferably aromatic amino acids such as phenylalanine, tryptophan, and tyrosine at positions P1 and P1'. Anti-Pepsin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research. Synonyms: ec 3.4.23.1 antibody, Pepsin A antibody, Pepsin a precursor antibody, Pepsin antibody, Pepsinogen 1 second locus antibody, Pepsinogen 3 group 1 antibody, Pepsinogen a3 antibody

ID gène: 396892

UniProt: P00791