BrdU anticorps

N° du produit ABIN1607682

N° du produit (Fournisseur): 200-301-h50

Détail du produit anti-BrdU anticorps

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BrdU est non-conjugé

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Dot Blot (DB)

N° du produit (Fournisseur): 200-301-h50

Fournisseur: Rockland

Fonction: BrdU Antibody

Réactivité croisée (Details): This antibody reacts strongly with BrdU.

Attributs du produit: Synonyms: mouse anti-BrdU Antibody, Bromodeoxyuridine, 5-bromo-2'-deoxyuridine, BrdU

Purification: Anti-BrdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-BrdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.

Immunogen Type: Other

Clone: 29G6-E8

Isotype: IgG1 lambda

Information d'application

Indications d'application:

Flow Cytometry Dilution: 1:50-1:100

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-BrdU Antibody has been tested as suitable for immunofluorescence and immunoblot assays. Antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Antibody will detect incorporated BrdU thymidine analog from replicated cells.

Western Blot Dilution: 1:2000 - 1:5000

ELISA Dilution: 1:2000 - 1:10000

IF Microscopy Dilution: 1:500-1:3000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store BrdU Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-BrdU (ABIN1607682)

Silva, Peyre, Adhikari, Tielens, Tanco, Van Damme, Magno, Krusy, Agirman, Magiera, Kessaris, Malgrange, Andrieux, Janke, Nguyen: "Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis." dans: Cell, Vol. 172, Issue 5, pp. 1063-1078.e19, (2019) (PubMed).

Wang, Shen, Cheng, Deng, Engelhardt, Yan, Qiu: "Parvovirus Expresses a Small Noncoding RNA That Plays an Essential Role in Virus Replication." dans: Journal of virology, Vol. 91, Issue 8, (2017) (PubMed).

Détails sur BrdU

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Autre désignation: BrdU (BrdU Produits)

Classe de substances: Chemical

Sujet: Background: Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog. BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and BrdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for BrdU are subsequently used to detect the incorporated BrdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.