CPB1 anticorps (Biotin)

N° du produit ABIN1607707

Détail du produit anti-CPB1 anticorps (Biotin)

Antigène: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Reactivité: Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CPB1 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): Cross reactivity against Carboxypeptidase B from other sources may occur but have not been specifically determined.

Pureté: Anti-CARBOXYPEPTIDASE B (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase B [Porcine Pancreas].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Carboxypeptidase B [Porcine Pancreas]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-CARBOXYPEPTIDASE B (RABBIT) Antibody has been assayed against 1.0 µg of Carboxypeptidase B in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur CPB1

Antigène: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Autre désignation: Carboxypeptidase B (CPB1 Produits)

Synonymes: anticorps wu:fb60d07, anticorps wu:fb69b08, anticorps CPB1, anticorps pCPB, anticorps CPB, anticorps PASP, anticorps PCPB, anticorps Cpb, anticorps 0910001A18Rik, anticorps 1810063F02Rik, anticorps 2210008M23Rik, anticorps AI504870, anticorps carboxypeptidase B1 (tissue), anticorps carboxypeptidase B1, anticorps carboxypeptidase B1 S homeolog, anticorps carboxypeptidase B, anticorps cpb1, anticorps CPB1, anticorps cpb1.S, anticorps CpipJ_CPIJ010799, anticorps CpipJ_CPIJ010801, anticorps VDBG_02663, anticorps LOC100562609, anticorps Cpb1

Sujet: A carboxypeptidase (EC number 3.4.16 - 3.4.18) is a protease enzyme that hydrolyzes (cleaves) a peptide bond at the carboxy-terminal (C-terminal) end of a protein or peptide. (Contrast with an aminopeptidase, which cleaves peptide bonds at the other end of the protein.) Humans, animals, and plants contain several types of carboxypeptidases that have diverse functions ranging from catabolism to protein maturation. Carboxypeptidase B is a carboxypeptidase that preferentially acts upon basic amino acids, such as arginine and lysine. This serum enzyme is also responsible for rapidly metabolizing the C5a protein into C5a des-Arg, with one less amino acid. Synonyms: CarboxypeptidaseB1 antibody, CP B1 antibody, CPB 1 antibody, CPB antibody, CPB1 antibody, DKFZp779K1333 antibody, Pancreas specific protein antibody, Pancreatic carboxypeptidase B1 antibody

ID gène: 397341

UniProt: P09955