Ovalbumin anticorps (Biotin)

N° du produit ABIN1607759

Détail du produit anti-Ovalbumin anticorps (Biotin)

Antigène: Ovalbumin (OVA)

Reactivité: Poulet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ovalbumin est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivities against Ovalbumin from other sources may occur but have not been specifically determined.

Pureté: Anti-Ovalbumin Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Ovalbumin [Hen Egg White]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Ovalbumin Antibody Biotin Conjugated is suitable for both ELISA, and for western blotting. In western blots anti-ovalbumin detects a single band of the expected apparent molecular weight. Researchers should determine optimal titers for applications that are not stated below.
ELISA Dilution: 1:30.000
Fluorochrome Protein Value: 10-20
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur Ovalbumin

Antigène: Ovalbumin (OVA)

Autre désignation: Ovalbumin (OVA Produits)

Synonymes: anticorps 1600019A21Rik, anticorps R86, anticorps SPI-CI, anticorps Spi10, anticorps ovalbumin, anticorps NK13, anticorps Spi12, anticorps BC052216, anticorps NK21, anticorps NK21L1, anticorps Spi13, anticorps NK26, anticorps Spi14, anticorps AW540195, anticorps AT2, anticorps Spi9, anticorps EID, anticorps SPI3B, anticorps Gm11396, anticorps OTTMUSG00000000720, anticorps SERPINB14, anticorps C76171, anticorps C76174, anticorps SPI3C, anticorps SPIC, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 9b, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 6b, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 9f, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 9e, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 9d, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 1, pseudogene, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 6e, anticorps ovalbumin (SERPINB14), anticorps serine (or cysteine) peptidase inhibitor, clade B, member 6c, anticorps Serpinb9b, anticorps Serpinb6b, anticorps Serpinb9f, anticorps Serpinb9e, anticorps Serpinb9d, anticorps Serpinb1-ps1, anticorps Serpinb6e, anticorps OVAL, anticorps Serpinb6c

Sujet: Ovalbumin Antibody Biotin Conjugated is specific for ovalbumin. Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65 % of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily. The function of ovalbumin is unknown, although it is presumed to be a storage protein or carrier protein. Synonyms: Ovalbumin, Egg albumin. Plakalbumin, Allergen Gal d II, Allergen=Gal d 2

ID gène: 396058

UniProt: P01012