Glucose Oxidase anticorps (Biotin)

N° du produit ABIN1607767

Détail du produit anti-Glucose Oxidase anticorps (Biotin)

Antigène: Glucose Oxidase (GOD)

Reactivité: Aspergillus niger

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Glucose Oxidase est conjugé à/à la Biotin

Application: ELISA, Western Blotting (WB)

Réactivité croisée (Details): Cross reactivity against Glucose Oxidase from other sources may occur but have not been specifically determined.

Pureté: Anti-GLUCOSE OXIDASE Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [A.niger].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Glucose Oxidase [A.niger]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-GLUCOSE OXIDASE Antibody has been assayed against 1.0 µg of Glucose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:50.000 to 1:250.000 of the reconstitution concentration is suggested for this product. Anti-GLUCOSE OXIDASE Antibody has been assayed against 1.0 µg of Glucose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:50.000 to 1:250.000 of the reconstitution concentration is suggested for this product. Specific condtions should be optimized by user.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur Glucose Oxidase

Antigène: Glucose Oxidase (GOD)

Autre désignation: Glucose Oxidase (GOD Produits)

Synonymes: anticorps LOC406081, anticorps GB19418, anticorps glucose oxidase, anticorps LOC406081, anticorps AFUA_2G14840, anticorps SS1G_10949, anticorps AOR_1_952194, anticorps AOR_1_786174, anticorps AOR_1_358034, anticorps CC1G_15386, anticorps VDBG_09040, anticorps VDBG_06502, anticorps SCHCODRAFT_113971

Sujet: Glucose Oxidase antibody detect the glucose oxidase protein. The glucose oxidase enzyme (GOx) (EC 1.1.3.4) is an oxido-reductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. In cells, it aids in breaking the sugar down into its metabolites. Glucose oxidase is widely used for the determination of free glucose in body fluids (diagnostics), in vegetal raw material, and in the food industry. GOx is a dimeric protein, the 3D structure of which has been elucidated. The active site where glucose binds is in a deep pocket. The enzyme, like many proteins that act outside of cells, is covered with carbohydrate chains. At pH 7, glucose exists in solution in cyclic hemiacetal form as 63.6 % β-D-glucopyranose and 36.4 % α-D-glucopyranose, the proportion of linear and furanose form being negligible. Anti-Glucose Oxidase antibody is ideal for investigators involved in glucose energy metabolism research. Synonyms: Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

ID gène: 2683500

UniProt: P13006