Acid Phosphatase anticorps (Biotin)

N° du produit ABIN1607769

Détail du produit anti-Acid Phosphatase anticorps (Biotin)

Antigène: Acid Phosphatase (ACP)

Reactivité: Potato

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Acid Phosphatase est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): Cross reactivity against Acid Phosphatase from other sources is unknown.

Purification: Acid Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Acid Phosphatase [Potato]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Acid Phosphatase Biotin Conjugated Antibody has been assayed against 1.0 µg of Acid Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:8.000 to 1:32.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:4.000 - 1:20.000
Western Blot Dilution: 1:500 - 1:2.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur Acid Phosphatase

Antigène: Acid Phosphatase (ACP)

Autre désignation: Acid Phosphatase (ACP Produits)

Synonymes: anticorps Acph, anticorps ACP, anticorps Acid phosphatase, anticorps acid phosphatase, anticorps cerebral palsy, ataxic 1, anticorps Dana\Acph, anticorps phoC, anticorps CPAT1

Sujet: Acid Phosphatase is an enzyme which facilitates hydrolysis of alcohol groups on phosphate esters. Anti-Acid Phosphatase antibodies are ideal for investigators interested in Cancer, Enyzme, Cell Biology, and Cell Cycle Research. Synonyms: Acid phosphatase 1 soluble antibody, Acid phosphatase of erythrocyte antibody, Adipocyte acid phosphatase antibody, Cytoplasmic phosphotyrosyl protein phosphatase antibody, HAAP antibody

UniProt: Q6J5M8